LOCUS       sequence-89159-e        2183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1405..1414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1411..2142
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attcggcagc
       61 acttttcttt cattgggggg aaggcgtgag gaaagtacca aacagcagca gacttttaaa
      121 ctttaaacag acaggtctga gagcctgaac tctcattttt cctttgactt cagcctccaa
      181 ggagttccac caactttggc gcgccggctt cactttcatt aagtgaaaga gaggtgccca
      241 gacatgggtg actggagtgc cttggggaag cttctggaca aggtccaagc ctactccacc
      301 gctggaggga aggtgtggct gtcagtgctc ttcatattca gaatcctgct cctggggaca
      361 gctgttgagt cagcttgggg tgatgaacag tctgcctttc gctgtaacac tcaacaacct
      421 ggctgcgaaa acgtctgcta tgacaagtcc ttccccatct ctcacgtgcg cttctgggtc
      481 cttcagatca tattcgtgtc tgtgcccaca ctcctgtact tggcccatgt gttctatgtg
      541 atgaggaagg aagagaagct aaacaagaaa gaagaggagc tcaaagtggc ccagactgac
      601 ggggtcaacg tggagatgca cctgaagcag attgaaatca agaagttcaa gtacgggatt
      661 gaagagcacg gcaaggtgaa aatgaggggc ggcttgctga gaacctacat catcagcatc
      721 ctcttcaagt ctgtcttcga ggtggccttc ctgctcatcc agtggtacat ctatgggttc
      781 agcttgagcg cggtctacac ctgcaagaga gatccctgcc cccaccaggt agactgcttc
      841 ctctcacgtc ccacggagaa aaccatcttc atcatcttca tgctggtggt gtccttggtg
      901 tctctcgctt tgaacatcat tgagctcttc tacgtcttct tcaaaggcgt taaggatcgc
      961 gtgaagggaa gaagcgatcc ttaccacgcc accactggcc cactgagccc atcaaaagac
     1021 tgcggatctc caaaatacgc ctacttcaat ggctgctcct caccaacggc tccactctcg
     1081 cctatgtctc ctcctgggta caagctggtt actggtgaca gaaacaattc ctcgtgccgc
     1141 aattacaaca agcaagctag cgagcaaaac tgggcgaact acagcgcaga gcaaaatcgc
     1201 atggggcagg ccggaagcac catctccaac tcgcacgccc agccgttcga tttccccgac
     1261 gacaaccaga atgccaaaaa agttgctgct ggacatgaac tccagccatt agccatcgtg
     1321 gaccaacgac cttccagcag agccagcagc cgcgccagca gcaggcctcg gcctgatgac
     1381 ctggagattg cggatccacc ggtcgccacc atggtgtcta agggcgaaga gctgattaag
     1441 gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc
     1501 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     1561 gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc
     1621 agaaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc cttccctgag
     1681 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     1741 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     1801 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccaa caccgagatg
     1861 ctgtaccccg ctgacggcgg cctggaaggc agaaccgaca tggccctgaa gctcgtgggc
     1921 gggggccacc tgatctgcaa cttcaagacc acatacagat ccaagaaacc cgctaagaac
     1981 ctcaagatgc ccggcgtcta ctatgtggac cacagactgg aaagaatcaa ggaggccgac
     2041 aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc
     2101 aaactggggc acaaacttaa tggcatggac gagctgtaca agtaagcggc cgcgactcta
     2161 gatcataatc agccatacca cat
//
