LOCUS       sequence-89162-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-ER-5
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..91
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     42..62
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      186..195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             192..923
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gggactttcc aaaaatgtcg taacaactcc gcccccattg acgcaaatgg gcggtaggcg
       61 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
      121 ccaccatgct gctatccgtg ccgttgctgc tcggcctcct cggcctggcc gtcgccgcac
      181 cggtcgccac catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc
      241 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca
      301 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
      361 tcgccttcga catcctggct accagcttca tgtacggcag cagaaccttc atcaaccaca
      421 cccagggcat ccccgatttc tttaagcagt ccttccctga gggcttcaca tgggagagag
      481 tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
      541 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga
      601 tgcagaagaa aacactcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg
      661 gcctggaagg cagaaccgac atggccctga agctcgtggg cgggggccac ctgatctgca
      721 acttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct
      781 actatgtgga ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc
      841 agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta
      901 atggcatgga cgagctgtac aagtccggaa aggacgagct gtaagaattc tgcagtcgac
      961 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
     1021 tttgtagagg ttttacttgc tttaaaaaat ctcccacacc tcccc
//
