LOCUS       sequence-89163-e        1491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mTagRFP-T-Endosomes-14
FEATURES             Location/Qualifiers
     source          1..1491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..761
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             807..836
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgtctaa gggcgaagag ctgattaagg
       61 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
      121 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
      181 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
      241 gaaccttcat caaccacacc cagggcatcc ccgatttctt taagcagtcc ttccctgagg
      301 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
      361 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc
      421 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagatgc
      481 tgtaccccgc tgacggcggc ctggaaggca gaaccgacat ggccctgaag ctcgtgggcg
      541 ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc
      601 tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca
      661 aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
      721 aactggggca caaacttaat ggcatggacg agctgtacaa gtccggatca gatctcgagc
      781 tcaagcttga tatcgaattc cccatggagc agaagctgat ctccgaggag gacctgaacc
      841 ggaagaaact ggtggttgtt ggtgatggag cctgtggaaa gacatgcttg ctcatagtct
      901 tcagcaagga cgagttccca gaggtgtatg tgcccacagt gtttgagaac tatgtggcag
      961 atatcgaggt ggatggaaag caggtagagt tggctttgtg ggacacagct ggccaggagg
     1021 actacgaccg cctgcggccg ctctcctacc cggacaccga cgtcattctc atgtgcttct
     1081 cggtggacag cccggactcg ctggagaaca tccccgagaa gtgggtcccc gaggtgaagc
     1141 acttctgtcc caatgtgccc atcatcctgg tggccaacaa aaaagacctg cgcagcgacg
     1201 agcatgtccg cacagagctg gcccgcatga agcaggaacc cgttcgcacg gatgacggcc
     1261 gcgccatggc cgtgcgcatc caagcctacg actacctcga gtgctctgcc aagaccaagg
     1321 aaggcgtgcg cgaggtcttc gagacggcca cgcgcgccgc gctgcagaag cgctacggct
     1381 cccagaacgg ctgcatcaac tgctgcaagg tgctatgagg gccgcgcccg tcgcgcctgc
     1441 ccctgccggg gatccaccgg atctagataa ctgatcataa tcagccatac c
//
