LOCUS       sequence-89166-e        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Gamma-Tubulin-17
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1423..1432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1429..2160
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgccg agggaaatca tcaccctaca gttgggccag
       61 tgcggcaatc agattgggtt cgagttctgg aaacagctgt gcgccgagca tggtatcagc
      121 cccgagggca tcgtggagga gttcgccacc gagggcactg accacaagga cgtctttttc
      181 taccaggcag acgatgagca ctacatcccc cgggccgtgc tgctggactt ggaaccccgg
      241 gtgatccact ccatcctcaa ctccccctat gccaagctct acaacccaga gaacatctac
      301 ctgtcggaac atggaggagg agctggcaac aactgggcca gcggattctc ccagggagaa
      361 aagatccatg aggacatttt tgacatcata gaccgggagg cagatggtag tgacagtcta
      421 gagggctttg tgctgtgtca ctccattgct ggggggacag gctctggact gggttcctac
      481 ctcttagaac ggctgaatga caggtatcct aagaagctgg tgcagacata ctcagtgttt
      541 cccaaccagg acgagatgag cgatgtggtg gtccagcctt acaattcact cctcacactc
      601 aagaggctga cgcagaatgc agactgtgtg gtggtgctgg acaacacagc cctgaaccgg
      661 attgccacag accgcctgca catccagaac ccatccttct cccagatcaa ccagctggtg
      721 tctaccatca tgtcagccag caccaccacc ctgcgctacc ctggctacat gaacaatgac
      781 ctcatcggcc tcatcgcctc gctcattccc accccacggc tccacttcct catgaccggc
      841 tacacccctc tcactacgga ccagtcagtg gccagcgtga ggaagaccac ggtcctggat
      901 gtcatgaggc ggctgctgca gcccaagaac gtgatggtgt ccacaggccg agaccgccag
      961 accaaccact gctacatcgc catcctcaac atcatccagg gagaggtgga ccccacccag
     1021 gtccacaaga gcttgcagag gatccgggaa cgcaagttgg ccaacttcat cccgtggggc
     1081 cccgccagca tccaggtggc cctgtcgagg aagtctccct acctgccctc ggcccaccgg
     1141 gtcagcgggc tcatgatggc caaccacacc agcatctcct cgctcttcga gagaacctgt
     1201 cgccagtatg acaagctgcg taagcgggag gccttcctgg agcagttccg caaggaggac
     1261 atgttcaagg acaactttga tgagatggac acatccaggg agattgtgca gcagctcatc
     1321 gatgagtacc atgcggccac acggccagac tacatctcct ggggcactca ggagcagcga
     1381 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgtctaag
     1441 ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
     1501 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     1561 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
     1621 agcttcatgt acggcagcag aaccttcatc aaccacaccc agggcatccc cgatttcttt
     1681 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     1741 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     1801 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
     1861 gaggccaaca ccgagatgct gtaccccgct gacggcggcc tggaaggcag aaccgacatg
     1921 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc
     1981 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa
     2041 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
     2101 tactgcgacc tccctagcaa actggggcac aaacttaatg gcatggacga gctgtacaag
     2161 taagcggccg cgactctaga tcataatcag ccataccaca t
//
