LOCUS       sequence-89167-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      295..304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             301..1032
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcatgaggc ttcgggagcc gctcctgagc
       61 ggcagcgccg cgatgccagg cgcgtcccta cagcgggcct gccgcctgct cgtggccgtc
      121 tgcgctctgc accttggcgt caccctcgtt tactacctgg ctggccgcga cctgagccgc
      181 ctgccccaac tggtcggagt ctccacaccg ctgcagggcg gctcgaacag tgccgccgcc
      241 atcgggcagt cctccgggga gctccggacc ggaggggcca aggatccacc ggtcgccacc
      301 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
      361 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      421 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      481 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaaccacac ccagggcatc
      541 cccgatttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      601 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
      661 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
      721 acactcggct gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctggaaggc
      781 agaaccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttcaagacc
      841 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
      901 cacagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg
      961 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa tggcatggac
     1021 gagctgtaca agtaagcggc cgcgactcta gatcataatc agcca
//
