LOCUS       sequence-89168-e        1207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-H2B-6
FEATURES             Location/Qualifiers
     source          1..1207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..440
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      453..462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             459..1190
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcca
       61 ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa gggctccaag aaggcggtga
      121 ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag agctattcca
      181 tctatgtgta caaggttctg aagcaggtcc accctgacac cggcatttcg tccaaggcca
      241 tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc
      301 gcctggcgca ttacaacaag cgctcgacca tcacctccag ggagatccag acggccgtgc
      361 gcctgctgct gcctggggag ttggccaagc acgccgtgtc cgagggtact aaggccatca
      421 ccaagtacac cagcgctaag gatccaccgg tcgccaccat ggtgtctaag ggcgaagagc
      481 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
      541 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
      601 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
      661 acggcagcag aaccttcatc aaccacaccc agggcatccc cgatttcttt aagcagtcct
      721 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
      781 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
      841 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccaaca
      901 ccgagatgct gtaccccgct gacggcggcc tggaaggcag aaccgacatg gccctgaagc
      961 tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc aagaaacccg
     1021 ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa agaatcaagg
     1081 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     1141 tccctagcaa actggggcac aaacttaatg gcatggacga gctgtacaag taagcggccg
     1201 cgactct
//
