LOCUS       sequence-89170-e        1345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1345)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      568..577
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             574..1305
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 ctagcgctac cggactcaga tctcgagctc aagcttatgt cgagcaagag agccaaggcc
       61 aagaccacca agaagaggcc ccagagggct acgtccaatg tcttcgccat gtttgaccag
      121 tcccagatcc aggagtttaa ggaggccttc aacatgattg atcagaaccg cgatggcttc
      181 attgataagg aggacctgca cgacatgttg gcctctctgg ggaagaaccc cacagacgag
      241 tacctggagg gcatgatgaa cgaggcgcca gggcccatca acttcaccat gttcctcaca
      301 atgtttgggg agaagctgaa cggcacagac cccgaggatg tgatccgcaa tgcctttgcc
      361 tgctttgatg aggaggcctc aggcttcatc cacgaggacc acctgaggga gctgctcacc
      421 accatgggcg accgattcac ggatgaggag gtggacgaga tgtaccgcga ggcacccatt
      481 gataagaagg gcaacttcaa ctacgtggag ttcactcgca tcctcaaaca cggcgccaag
      541 gacaaggacg acctggatcc accggtcgcc accatggtgt ctaagggcga agagctgatt
      601 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
      661 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
      721 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
      781 agcagaacct tcatcaacca cacccagggc atccccgatt tctttaagca gtccttccct
      841 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
      901 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
      961 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc caacaccgag
     1021 atgctgtacc ccgctgacgg cggcctggaa ggcagaaccg acatggccct gaagctcgtg
     1081 ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa acccgctaag
     1141 aacctcaaga tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaggaggcc
     1201 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     1261 agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagtaagc ggccgcgact
     1321 ctagatcata atcagccata ccaca
//
