LOCUS       sequence-89171-e        2097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(43..105,106..1263)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1318..1327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1324..2055
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgagcgcca ccatggcggc cccgggcgcc
       61 cggcgtccgc tgctcctgtt gctgctggca ggccttgcac acagcgcccc agcactgttc
      121 gaggtgaaag acaacaacgg cacagcgtgt ataatggcca gcttctctgc ctcctttctg
      181 accacctatg aggctggaca tgtttctaag gtctcgaata tgaccctgcc agcctctgca
      241 gaagtcctga agaatagcag ctcttgtggt gaaaagaatg cttctgagcc caccctcgca
      301 atcacctttg gagaaggata tttactgaaa ctcaccttca caaaaaacac aacacgttac
      361 agtgtccagc acatgtattt cacatataac ctgtcagaca cacaattctt tcccaatgcc
      421 agctccaaag ggcccgacac tgtggattcc acaactgaca tcaaggcaga catcaacaaa
      481 acataccgat gtgtcagcga catcagggtc tacatgaaga atgtgaccat tgtgctctgg
      541 gacgctacta tccaggccta cctgccgagt agcaacttca gcaaggaaga gacacgctgc
      601 ccacaggatc aaccttcccc aactactggg ccacccagcc cctcaccacc acttgtgccc
      661 acaaacccca gtgtgtccaa gtacaatgtg actggtgaca atggaacctg cctgctggcc
      721 tctatggcac tgcaactcaa catcacctac atgaagaagg acaacacgac tgtgaccaga
      781 gcattcaaca tcaacccaag tgacaaatat agtgggactt gcggtgccca gttggtgacc
      841 ctgaaggtgg ggaacaagag cagagtcctg gagctgcagt ttgggatgaa tgccacttct
      901 agcctgtttt tcctgcaagg agttcagttg aacatgactc ttcctgatgc catagagccc
      961 acgttcagca cctccaacta ttccctgaaa gctcttcagg ccagtgtcgg caactcatac
     1021 aagtgcaaca gtgaggagca catctttgtc agcaaggcgc tcgccctcaa tgtcttcagc
     1081 gtgcaagtcc aggctttcag ggtagaaagt gacaggtttg ggtctgtgga agagtgtgta
     1141 caggacggta acaacatgct gatccccatt gctgtgggcg gggccctggc agggctggtc
     1201 ctcatcgtcc tcatcgccta cctcatcggc aggaagagga gtcacgcggg ctatcagacc
     1261 atctcggaat tcggctccac cggctccacc ggctccaccg gcgcggatcc accggtcgcc
     1321 accatggtgt ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg
     1381 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
     1441 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     1501 gacatcctgg ctaccagctt catgtacggc agcagaacct tcatcaacca cacccagggc
     1561 atccccgatt tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca
     1621 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     1681 atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag
     1741 aaaacactcg gctgggaggc caacaccgag atgctgtacc ccgctgacgg cggcctggaa
     1801 ggcagaaccg acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttcaag
     1861 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg
     1921 gaccacagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
     1981 gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact taatggcatg
     2041 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatt
//
