LOCUS       sequence-89174-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    189..241
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      249..258
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             255..986
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 agcgccgcgg ctcgctgtgc tctggctgcc agaagcccat cacgggacgc tgcatcactg
       61 ccatgggcaa gaaatttcac cccgaacact ttgtctgtgc cttctgcctc aagcagctca
      121 acaaaggaac cttcaaagaa cagaacgaca agccctactg tcagaactgc tttctcaaac
      181 tcttctgtct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      241 caccggtcgc caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga
      301 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
      361 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
      421 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaacc
      481 acacccaggg catccccgat ttctttaagc agtccttccc tgagggcttc acatgggaga
      541 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
      601 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg
      661 tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac cccgctgacg
      721 gcggcctgga aggcagaacc gacatggccc tgaagctcgt gggcgggggc cacctgatct
      781 gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg
      841 tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
      901 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac
      961 ttaatggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat
     1021 accacatt
//
