LOCUS       sequence-89175-e        1490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1490)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..766
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg tctaagggcg aagagctgat
       61 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
      121 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
      181 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
      241 cagcagaacc ttcatcaacc acacccaggg catccccgat ttctttaagc agtccttccc
      301 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
      361 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
      421 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga
      481 gatgctgtac cccgctgacg gcggcctgga aggcagaacc gacatggccc tgaagctcgt
      541 gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa
      601 gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc
      661 cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
      721 tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtccg gactcagatc
      781 tcgagccatg tcgcagacgg ccatgtccga aacctacgat tttttgttta agttcttggt
      841 tattggaaat gcaggaactg gcaaatcttg cttacttcat cagtttattg aaaaaaaatt
      901 caaagatgac tcaaatcata caataggagt ggaatttggt tcaaagataa taaatgttgg
      961 tggtaaatat gtaaagttac aaatatggga tacagcagga caagaacgat tcaggtccgt
     1021 gacgagaagt tattaccgag gcgcggccgg ggctctcctc gtctatgata tcaccagccg
     1081 agaaacctac aatgcgctta ctaattggtt aacagatgcc cgaatgctgg cgagccagaa
     1141 cattgtgatc atcctttgtg gaaacaagaa ggacctggat gcagatcgtg aagttacctt
     1201 cttagaagcc tccagatttg ctcaagaaaa tgagctgatg tttttggaaa caagtgcgct
     1261 cacaggggag aatgtagaag aggcttttgt acagtgtgca agaaaaatac ttaacaaaat
     1321 cgaatcaggt gagctggacc cagaaagaat gggctcaggt attcagtacg gagatgctgc
     1381 cttgagacag ctgaggtcac cgcggcgcgc acaggccccg aacgctcagg agtgtggttg
     1441 ttagggatcc accggatcta gataactgat cataatcagc cataccacat
//
