LOCUS       sequence-89176-e        2182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2182)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..763
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgtct aagggcgaag agctgattaa
       61 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg
      121 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
      181 cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag
      241 cagaaccttc atcaaccaca cccagggcat ccccgatttc tttaagcagt ccttccctga
      301 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
      361 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
      421 cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca acaccgagat
      481 gctgtacccc gctgacggcg gcctggaagg cagaaccgac atggccctga agctcgtggg
      541 cgggggccac ctgatctgca acttcaagac cacatacaga tccaagaaac ccgctaagaa
      601 cctcaagatg cccggcgtct actatgtgga ccacagactg gaaagaatca aggaggccga
      661 caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag
      721 caaactgggg cacaaactta atggcatgga cgagctgtac aagtccggac tcagatctcg
      781 agtgcgtgag tgcatctcca tccacgttgg ccaggctggt gtccagattg gcaatgcctg
      841 ctgggagctc tactgcctgg aacacggcat ccagcccgat ggccagatgc caagtgacaa
      901 gaccattggg ggaggagatg actccttcaa caccttcttc agtgagacgg gcgctggcaa
      961 gcacgtgccc cgggctgtgt ttgtagactt ggaacccaca gtcattgatg aagttcgcac
     1021 tggcacctac cgccagctct tccaccctga gcagctcatc acaggcaagg aagatgctgc
     1081 caataactat gcccgagggc actacaccat tggcaaggag atcattgacc ttgtgttgga
     1141 ccgaattcgc aagctggctg accagtgcac cggtcttcag ggcttcttgg ttttccacag
     1201 ctttggtggg ggaactggtt ctgggttcac ctccctgctc atggaacgtc tctcagttga
     1261 ttatggcaag aagtccaagc tggagttctc catttaccca gcaccccagg tttccacagc
     1321 tgtagttgag ccctacaact ccatcctcac cacccacacc accctggagc actctgattg
     1381 tgccttcatg gtagacaatg aggccatcta tgacatctgt cgtagaaacc tcgatatcga
     1441 gcgcccaacc tacactaacc ttaaccgcct tattagccag attgtgtcct ccatcactgc
     1501 ttccctgaga tttgatggag ccctgaatgt tgacctgaca gaattccaga ccaacctggt
     1561 gccctacccc cgcatccact tccctctggc cacatatgcc cctgtcatct ctgctgagaa
     1621 agcctaccat gaacagcttt ctgtagcaga gatcaccaat gcttgctttg agccagccaa
     1681 ccagatggtg aaatgtgacc ctcgccatgg taaatacatg gcttgctgcc tgttgtaccg
     1741 tggtgacgtg gttcccaaag atgtcaatgc tgccattgcc accatcaaaa ccaagcgcag
     1801 catccagttt gtggattggt gccccactgg cttcaaggtt ggcatcaact accagcctcc
     1861 cactgtggtg cctggtggag acctggccaa ggtacagaga gctgtgtgca tgctgagcaa
     1921 caccacagcc attgctgagg cctgggctcg cctggaccac aagtttgacc tgatgtatgc
     1981 caagcgtgcc tttgttcact ggtacgtggg tgaggggatg gaggaaggcg agttttcaga
     2041 ggcccgtgaa gatatggctg cccttgagaa ggattatgag gaggttggtg tggattctgt
     2101 tgaaggagag ggtgaggaag aaggagagga atactaagga tccaccggat ctagataact
     2161 gatcataatc agccatacca ca
//
