LOCUS       sequence-89177-e        1944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1944)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-VASP-5
FEATURES             Location/Qualifiers
     source          1..1944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..764
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgtc taagggcgaa gagctgatta
       61 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
      121 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
      181 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
      241 gcagaacctt catcaaccac acccagggca tccccgattt ctttaagcag tccttccctg
      301 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
      361 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
      421 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc aacaccgaga
      481 tgctgtaccc cgctgacggc ggcctggaag gcagaaccga catggccctg aagctcgtgg
      541 gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa cccgctaaga
      601 acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc aaggaggccg
      661 acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
      721 gcaaactggg gcacaaactt aatggcatgg acgagctgta caagtccgga ctcagatcta
      781 tgagcgagac ggtcatctgt tccagccggg ctactgtgat gctttatgat gacagcaaca
      841 agcgatggct ccctgctggc actggtcccc aggccttcag ccgcgtccag atataccaca
      901 accccactgc taactccttc cgagttgttg gccgcaagat gcagccggat cagcaggtgg
      961 ttatcaactg tgccatcatt cggggtgtca agtacaatca ggccactccc atcttccatc
     1021 agtggcgaga tgcccgccag gtctggggcc tcaacttcgg cagcaaggag gacgccatac
     1081 agtttgcaac aggcatggcc aacgccctag aggccttgga aggaggtggg cctcccccag
     1141 ccccagcacc ccctgcctgg tctgcccaga atggtccctc cccagaggag ctggaacaac
     1201 agaaaaggca gccggagcat atggagcgcc gggtctccaa tgcaggaggc ccacctgctc
     1261 ccccagctgg gggccctcct ccacctccag gacctccccc tcctccaggt ccccccccac
     1321 ccccaggtct gccctcctca ggggtatctg gggcaggtca tggagcaggg gcagccccac
     1381 cccctgcacc cccactccct acaacacagg gccccaatag tgggggttcc ggggccccag
     1441 gcctggctgc tgccattgct ggagccaaac tcaggaaagt gagcaagcag gaggaggcct
     1501 ctggggggcc cctggccccc aaagctgaga acagtcgaag cactggtggg gggcttatgg
     1561 aagagatgaa cgccatgctg gcccggagaa gaaaagccac acaggttggg gagaagcccc
     1621 ccaaagacga gtcagccagt gaggagtcag aggcccgact ccctgcccag agtgaacctg
     1681 tgagaagacc ctgggagaag aacagcacaa ccttgccaag gatgaagtcg tcctcttctg
     1741 tgactacctc cgaggcccac ccctcgacgc cctgctccag tgatgactcc gacttggaga
     1801 gggtgaagca ggagcttctg gaagaggtgc ggaaggagct acagaaaatg aaagaggaaa
     1861 tcatcgaagt ctttgtccag gagctgagga agcggggttc tccttgagcg gccgcgactc
     1921 tagatcataa tcagccatac caca
//
