LOCUS       sequence-89179-e        2252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 555, Emission: 
            584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1470..1479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1476..2207
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attcccatgt
       61 ccaccaggtc cgtgtcctcg tcctcctacc gcaggatgtt cggcggcccg ggcaccgcga
      121 gccggccgag ctccagccgg agctacgtga ctacgtccac ccgcacctac agcctgggca
      181 gcgcgctgcg ccccagcacc agccgcagcc tctacgcctc gtccccgggc ggcgtgtatg
      241 ccacgcgctc ctctgccgtg cgcctgcgga gcagcgtgcc cggggtgcgg ctcctgcagg
      301 actcggtgga cttctcgctg gccgacgcca tcaacaccga gttcaagaac acccgcacca
      361 acgagaaggt ggagctgcag gagctgaatg accgcttcgc caactacatc gacaaggtgc
      421 gcttcctgga gcagcagaat aagatcctgc tggccgagct cgagcagctc aagggccaag
      481 gcaagtcgcg cctgggggac ctctacgagg aggagatgcg ggagctgcgc cggcaggtgg
      541 accagctaac caacgacaaa gcccgcgtcg aggtggagcg cgacaacctg gccgaggaca
      601 tcatgcgcct ccgggagaaa ttgcaggagg agatgcttca gagagaggaa gccgaaaaca
      661 ccctgcaatc tttcagacag gatgttgaca atgcgtctct ggcacgtctt gaccttgaac
      721 gcaaagtgga atctttgcaa gaagagattg cctttttgaa gaaactccac gaagaggaaa
      781 tccaggagct gcaggctcag attcaggaac agcatgtcca aatcgatgtg gatgtttcca
      841 agcctgacct cacggctgcc ctgcgtgacg tacgtcagca atatgaaagt gtggctgcca
      901 agaacctgca ggaggcagaa gaatggtaca aatccaagtt tgctgacctc tctgaggctg
      961 ccaaccggaa caatgacgcc ctgcgccagg caaagcagga gtccactgag taccggagac
     1021 aggtgcagtc cctcacctgt gaagtggatg cccttaaagg aaccaatgag tccctggaac
     1081 gccagatgcg tgaaatggaa gagaactttg ccgttgaagc tgctaactac caagacacta
     1141 ttggccgcct gcaggatgag attcagaata tgaaggagga aatggctcgt caccttcgtg
     1201 aataccaaga cctgctcaat gttaagatgg cccttgacat tgagattgcc acctacagga
     1261 agctgctgga aggcgaggag agcaggattt ctctgcctct tccaaacttt tcctccctga
     1321 acctgaggga aactaatctg gattcactcc ctctggttga tacccactca aaaaggacac
     1381 ttctgattaa gacggttgaa actagagatg gacaggttat caacgaaact tctcagcatc
     1441 acgatgacct tgaaggggat ccaccggtcg ccaccatggt gtctaagggc gaagagctga
     1501 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
     1561 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
     1621 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
     1681 gcagcagaac cttcatcaac cacacccagg gcatccccga tttctttaag cagtccttcc
     1741 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
     1801 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
     1861 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gccaacaccg
     1921 agatgctgta ccccgctgac ggcggcctgg aaggcagaac cgacatggcc ctgaagctcg
     1981 tgggcggggg ccacctgatc tgcaacttca agaccacata cagatccaag aaacccgcta
     2041 agaacctcaa gatgcccggc gtctactatg tggaccacag actggaaaga atcaaggagg
     2101 ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc
     2161 ctagcaaact ggggcacaaa cttaatggca tggacgagct gtacaagtaa gcggccgcga
     2221 ctctagatca taatcagcca taccacattg ag
//
