LOCUS       sequence-89181-e        2561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2561)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1782..1791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1788..2519
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat tccatggcgg
       61 ccccccgccc gtctcccgcg atccccgttt cggtctcggc tccggctttt tacgccccgc
      121 agaagaagtt cggccctgtg gtggccccaa agcccaaagt gaatcccttc cggcccaggg
      181 acagcgagcc tcccccggca cccggggccc agcgcgcaca gatgggccgg gtgggcgata
      241 ttcccccgcc gcccccggaa gactttcccc tgcctccacc tccccttgct ggggatggcg
      301 acgatgcaga gggtgctctg ggaggtgcct tcccgccgcc ccctcccccg atcgaggaat
      361 catttccccc tgcgcctctg gaggaggaga tcttcccttc cccgccgcct cctccggagg
      421 aggagggagg gcctgaggcc cccataccgc ccccaccaca gcccagggag aaggtgagca
      481 gtattgattt ggagatcgac tctctgtcct cactgctgga tgacatgacc aagaatgatc
      541 ctttcaaagc ccgggtgtca tctggatatg tgcccccacc agtggccact ccattcagtt
      601 ccaagtccag taccaagcct gcagccgggg gcacagcacc cctgcctcct tggaagtccc
      661 cttccagctc ccagcctctg ccccaggttc cggctccggc tcagagccag acacagttcc
      721 atgttcagcc ccagccccag cccaagcctc aggtccaact ccatgtccag tcccagaccc
      781 agcctgtgtc tttggctaac acccagcccc gagggccccc agcctcatct ccggctccag
      841 cccctaagtt ttctccagtg actcctaagt ttactcctgt ggcttccaag ttcagtcctg
      901 gagccccagg tggatctggg tcacaaccaa atcaaaaatt ggggcacccc gaagctcttt
      961 ctgctggcac aggctcccct caacctccca gcttcaccta tgcccagcag agggagaagc
     1021 cccgagtgca ggagaagcag caccccgtgc ccccaccggc tcagaaccaa aaccaggtgc
     1081 gctcccctgg ggccccaggg cccctgactc tgaaggaggt ggaggagctg gagcagctga
     1141 cccagcagct aatgcaggac atggagcatc ctcagaggca gaatgtggct gtcaacgaac
     1201 tctgcggccg atgccatcaa cccctggccc gggcgcagcc agccgtccgc gctctagggc
     1261 agctgttcca catcgcctgc ttcacctgcc accagtgtgc gcagcagctc cagggccagc
     1321 agttctacag tctggagggg gcgccgtact gcgagggctg ttacactgac accctggaga
     1381 agtgtaacac ctgcggggag cccatcactg accgcatgct gagggccacg ggcaaggcct
     1441 atcacccgca ctgcttcacc tgtgtggtct gcgcccgccc cctggagggc acctccttca
     1501 tcgtggacca ggccaaccgg ccccactgtg tccccgacta ccacaagcag tacgccccga
     1561 ggtgctccgt ctgctctgag cccatcatgc ctgagcctgg ccgagatgag actgtgcgag
     1621 tggtcgccct ggacaagaac ttccacatga agtgttacaa gtgtgaggac tgcgggaagc
     1681 ccctgtcgat tgaggcagat gacaatggct gcttccccct ggacggtcac gtgctctgtc
     1741 ggaagtgcca cactgctaga gcccagacgg atccaccggt cgccaccatg gtgtctaagg
     1801 gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
     1861 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     1921 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
     1981 gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc gatttcttta
     2041 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     2101 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     2161 tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     2221 aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga accgacatgg
     2281 ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca
     2341 agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa
     2401 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat
     2461 actgcgacct ccctagcaaa ctggggcaca aacttaatgg catggacgag ctgtacaagt
     2521 aagcggccgc gactctagat cataatcagc cataccacat t
//
