LOCUS       sequence-89185-e        2691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2691)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Actin-7
FEATURES             Location/Qualifiers
     source          1..2691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..728
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             798..1490
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 gaacgtcaga tccgctagcg ctaccggtcg ccaccatgag cgagctgatc aaggagaaca
       61 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
      121 agggcgaagg caagccctac gagggcaccc agaccatgaa gatcaaggtg gtcgagggcg
      181 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaagcct
      241 tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct gagggcttca
      301 catgggagag aatcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
      361 gcttccagaa cggctgcatc atctacaacg tcaagatcaa cggggtgaac ttcccatcca
      421 acggccctgt gatgcagaag aaaacacgcg gctgggaggc caacaccgag atgctgtacc
      481 ccgctgacgg cggcctgaga ggccacagcc agatggccct gaagctcgtg ggcgggggct
      541 acctgcactg ctccttcaag accacataca gatccaagaa acccgctaag aacctcaaga
      601 tgcccggctt ccacttcgtg gaccacagac tggaaagaat caaggaggcc gacaaagaga
      661 cctacgtcga gcagcacgag atggctgtgg ccaagtactg cgacctccct agcaaactgg
      721 ggcacagagg gcatggcacc ggcagcaccg gcagcggcag ctccggtacc gcctcctccg
      781 aggacaacaa catggccatg agcgagctga tcaaggagaa catgcacatg aagctgtaca
      841 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
      901 acgagggcac ccagaccatg aagatcaagg tggtcgaggg cggccctctc cccttcgcct
      961 tcgacatcct ggctaccagc ttcatgtacg gcagcaaagc cttcatcaac cacacccagg
     1021 gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag agaatcacca
     1081 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcttccag aacggctgca
     1141 tcatctacaa cgtcaagatc aacggggtga acttcccatc caacggccct gtgatgcaga
     1201 agaaaacacg cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctga
     1261 gaggccacag ccagatggcc ctgaagctcg tgggcggggg ctacctgcac tgctccttca
     1321 agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc ttccacttcg
     1381 tggaccacag actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg
     1441 agatggctgt ggccaagtac tgcgacctcc ctagcaaact ggggcacaga gatgaatccg
     1501 gactcagatc tcgagctatg gatgatgata tcgccgcgct cgtcgtcgac aacggctccg
     1561 gcatgtgcaa ggccggcttc gcgggcgacg atgccccccg ggccgtcttc ccctccatcg
     1621 tggggcgccc caggcaccag ggcgtgatgg tgggcatggg tcagaaggat tcctatgtgg
     1681 gcgacgaggc ccagagcaag agaggcatcc tcaccctgaa gtaccccatc gagcacggca
     1741 tcgtcaccaa ctgggacgac atggagaaaa tctggcacca caccttctac aatgagctgc
     1801 gtgtggctcc cgaggagcac cccgtgctgc tgaccgaggc ccccctgaac cccaaggcca
     1861 accgcgagaa gatgacccag atcatgtttg agaccttcaa caccccagcc atgtacgttg
     1921 ctatccaggc tgtgctatcc ctgtacgcct ctggccgtac cactggcatc gtgatggact
     1981 ccggtgacgg ggtcacccac actgtgccca tctacgaggg gtatgccctc ccccatgcca
     2041 tcctgcgtct ggacctggct ggccgggacc tgactgacta cctcatgaag atcctcaccg
     2101 agcgcggcta cagcttcacc accacggccg agcgggaaat cgtgcgtgac attaaggaga
     2161 agctgtgcta cgtcgccctg gacttcgagc aagagatggc cacggctgct tccagctcct
     2221 ccctggagaa gagctacgag ctgcctgacg gccaggtcat caccattggc aatgagcggt
     2281 tccgctgccc tgaggcactc ttccagcctt ccttcctggg catggagtcc tgtggcatcc
     2341 acgaaactac cttcaactcc atcatgaagt gtgacgtgga catccgcaaa gacctgtacg
     2401 ccaacacagt gctgtctggc ggcaccacca tgtaccctgg cattgccgac aggatgcaga
     2461 aggagatcac tgccctggca cccagcacaa tgaagatcaa gatcattgct cctcctgagc
     2521 gcaagtactc cgtgtggatc ggcggctcca tcctggcctc gctgtccacc ttccagcaga
     2581 tgtggatcag caagcaggag tatgacgagt ccggcccctc catcgtccac cgcaaatgct
     2641 tctagggatc caccggatct agataactga tcataatcag ccataccaca t
//
