LOCUS       sequence-89186-e        4297 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4297)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..4297
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2781..3473
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             3543..4235
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 cgctagcgct accggactca gatctcgagc tcaagcttcg caccatcatg gaccattatg
       61 attctcagca aaccaacgat tacatgcagc cagaagagga ctgggaccgg gacctgctcc
      121 tggacccggc ctgggagaag cagcagagaa agacattcac ggcatggtgt aactcccacc
      181 tccggaaggc ggggacacag atcgagaaca tcgaagagga cttccgggat ggcctgaagc
      241 tcatgctgct gctggaggtc atctcaggtg aacgcttggc caagccagag cgaggcaaga
      301 tgagagtgca caagatctcc aacgtcaaca aggccctgga tttcatagcc agcaaaggcg
      361 tcaaactggt gtccatcgga gccgaagaaa tcgtggatgg gaatgtgaag atgaccctgg
      421 gcatgatctg gaccatcatc ctgcgctttg ccatccagga catctccgtg gaagagactt
      481 cagccaagga agggctgctc ctgtggtgtc agagaaagac agccccttac aaaaatgtca
      541 acatccagaa cttccacata agctggaagg atggcctcgg cttctgtgct ttgatccacc
      601 gacaccggcc cgagctgatt gactacggga agctgcggaa ggatgatcca ctcacaaatc
      661 tgaatacggc ttttgacgtg gcagagaagt acctggacat ccccaagatg ctggatgccg
      721 aagacatcgt tggaactgcc cgaccggatg agaaagccat catgacttac gtgtctagct
      781 tctaccacgc cttctctgga gcccagaagg cggagacagc agccaatcgc atctgcaagg
      841 tgttggccgt caaccaggag aacgagcagc ttatggaaga ctacgagaag ctggccagtg
      901 atctgttgga gtggatccgc cgcacaatcc cgtggctgga gaaccgggtg cccgagaaca
      961 ccatgcatgc catgcaacag aagctggagg acttccggga ctaccggcgc ctgcacaagc
     1021 cgcccaaggt gcaggagaag tgccagctgg agatcaactt caacacgctg cagaccaagc
     1081 tgcggctcag caaccggcct gccttcatgc cctctgaggg caggatggtc tcggacatca
     1141 acaatgcctg gggctgcctg gagcaggtgg agaagggcta tgaggagtgg ttgctgaatg
     1201 agatccggag gctggagcga ctggaccacc tggcagagaa gttccggcag aaggcctcca
     1261 tccacgaggc ctggactgac ggcaaagagg ccatgctgcg acagaaggac tatgagaccg
     1321 ccaccctctc ggagatcaag gccttgctca agaagcatga ggccttcgag agtgacctgg
     1381 ctgcccacca ggaccgtgtg gagcagattg ccgccatcgc acaggagctc aatgagctgg
     1441 actattatga ctcacccagt gtcaacgccc gttgccaaaa gatctgtgac cagtgggaca
     1501 atctgggggc cctaactcag aagcgaaggg aagctctgga gcggaccgag aaactgctgg
     1561 agaccattga ccagctgtac ttggagtatg ccaagcgggc tgcacccttc aacaactgga
     1621 tggagggggc catggaggac ctgcaggaca ccttcattgt gcacaccatt gaggagatcc
     1681 agggactgac cacagcccat gagcagttca aggccaccct ccctgatgcc gacaaggagc
     1741 gcctggccat cctgggcatc cacaatgagg tgtccaagat tgtccagacc taccacgtca
     1801 atatggcggg caccaacccc tacacaacca tcacgcctca ggagatcaat ggcaaatggg
     1861 accacgtgcg gcagctggtg cctcggaggg accaagctct gacggaggag catgcccgac
     1921 agcagcacaa tgagaggcta cgcaagcagt ttggagccca ggccaatgtc atcgggccct
     1981 ggatccagac caagatggag gagatcggga ggatctccat tgagatgcat gggaccctgg
     2041 aggaccagct cagccacctg cggcagtatg agaagagcat cgtcaactac aagccaaaga
     2101 ttgatcagct ggagggcgac caccagctca tccaggaggc gctcatcttc gacaacaagc
     2161 acaccaacta caccatggag cacatccgtg tgggctggga gcagctgctc accaccatcg
     2221 ccaggaccat caatgaggta gagaaccaga tcctgacccg ggatgccaag ggcatcagcc
     2281 aggagcagat gaatgagttc cgggcctcct tcaaccactt tgaccgggat cactccggca
     2341 cactgggtcc cgaggagttc aaagcctgcc tcatcagctt gggttatgat attggcaacg
     2401 acccccaggg agaagcagaa tttgcccgca tcatgagcat tgtggacccc aaccgcctgg
     2461 gggtagtgac attccaggcc ttcattgact tcatgtcccg cgagacagcc gacacagata
     2521 cagcagacca agtcatggct tccttcaaga tcctggctgg ggacaagaac tacattacca
     2581 tggacgagct gcgccgcgag ctgccacccg accaggctga gtactgcatc gcgcggatgg
     2641 ccccctacac cggccccgac tccgtgccag gtgctctgga ctacatgtcc ttctccacgg
     2701 cgctgtacgg cgagagtgac ctcaagcttc gaattctgca gtcgacggta ccgcgggccc
     2761 gggatccacc ggtcgccacc atgagcgagc tgatcaagga gaacatgcac atgaagctgt
     2821 acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc
     2881 cctacgaggg cacccagacc atgaagatca aggtggtcga gggcggccct ctccccttcg
     2941 ccttcgacat cctggctacc agcttcatgt acggcagcaa agccttcatc aaccacaccc
     3001 agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg gagagaatca
     3061 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcttc cagaacggct
     3121 gcatcatcta caacgtcaag atcaacgggg tgaacttccc atccaacggc cctgtgatgc
     3181 agaagaaaac acgcggctgg gaggccaaca ccgagatgct gtaccccgct gacggcggcc
     3241 tgagaggcca cagccagatg gccctgaagc tcgtgggcgg gggctacctg cactgctcct
     3301 tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc ggcttccact
     3361 tcgtggacca cagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc
     3421 acgagatggc tgtggccaag tactgcgacc tccctagcaa actggggcac agagggcatg
     3481 gcaccggcag caccggcagc ggcagctccg gtaccgcctc ctccgaggac aacaacatgg
     3541 ccatgagcga gctgatcaag gagaacatgc acatgaagct gtacatggag ggcaccgtga
     3601 acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     3661 ccatgaagat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     3721 ccagcttcat gtacggcagc aaagccttca tcaaccacac ccagggcatc cccgacttct
     3781 ttaagcagtc cttccctgag ggcttcacat gggagagaat caccacatac gaagacgggg
     3841 gcgtgctgac cgctacccag gacaccagct tccagaacgg ctgcatcatc tacaacgtca
     3901 agatcaacgg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acacgcggct
     3961 gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctgagaggc cacagccaga
     4021 tggccctgaa gctcgtgggc gggggctacc tgcactgctc cttcaagacc acatacagat
     4081 ccaagaaacc cgctaagaac ctcaagatgc ccggcttcca cttcgtggac cacagactgg
     4141 aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgagatg gctgtggcca
     4201 agtactgcga cctccctagc aaactggggc acagagatga atccggactc agatcttgag
     4261 cggccgcgac tctagatcat aatcagccat accacat
//
