LOCUS       sequence-89192-e        2434 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 553, Emission: 
            576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2434)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-EB3-7
FEATURES             Location/Qualifiers
     source          1..2434
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             914..1606
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             1676..2368
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 ggactcagat ctcgagctca agcttcgaat tctgcagtcg actgccacca tggccgtcaa
       61 tgtgtactcc acatctgtga ccagtgaaaa tctgagtcgc catgatatgc ttgcatgggt
      121 caacgactcc ctgcacctca actataccaa gatagaacag ctttgttcag gggcagccta
      181 ctgccagttc atggacatgc tcttccccgg ctgtgtgcac ttgaggaaag tgaagttcca
      241 ggccaaacta gagcatgaat acatccacaa cttcaaggtg ctgcaagcag ctttcaagaa
      301 gatgggtgtt gacaaaatca ttcctgtaga gaaattagtg aaaggaaaat tccaagataa
      361 ttttgagttt attcagtggt ttaagaaatt ctttgacgca aactatgatg gaaaggatta
      421 caaccctctg ctggcgcggc agggccagga cgtagcgcca cctcctaacc caggtgatca
      481 gatcttcaac aaatccaaga aactcattgg cacagcagtt ccacagagga cgtcccccac
      541 aggcccaaaa aacatgcaga cctctggccg gctgagcaat gtggcccccc cctgcattct
      601 ccggaagaat cctccatcag cccgaaatgg cggccatgag actgatgccc aaattcttga
      661 actcaaccaa cagctggtgg acttgaagct gacagtggat gggctggaga aggaacgtga
      721 cttctacttc agcaaacttc gtgacatcga gctcatctgc caggagcatg aaagtgaaaa
      781 cagccctgtt atctcaggca tcattggcat cctctatgcc acagaggaag gattcgcacc
      841 ccctgaggac gatgagattg aagagcatca acaagaagac caggacgagt acctggatcc
      901 accggtcgcc accatgagcg agctgatcaa ggagaacatg cacatgaagc tgtacatgga
      961 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
     1021 gggcacccag accatgaaga tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     1081 catcctggct accagcttca tgtacggcag caaagccttc atcaaccaca cccagggcat
     1141 ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagaa tcaccacata
     1201 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ttccagaacg gctgcatcat
     1261 ctacaacgtc aagatcaacg gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     1321 aacacgcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctgagagg
     1381 ccacagccag atggccctga agctcgtggg cgggggctac ctgcactgct ccttcaagac
     1441 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcttcc acttcgtgga
     1501 ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgagat
     1561 ggctgtggcc aagtactgcg acctccctag caaactgggg cacagagggc atggcaccgg
     1621 cagcaccggc agcggcagct ccggtaccgc ctcctccgag gacaacaaca tggccatgag
     1681 cgagctgatc aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca
     1741 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgaa
     1801 gatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
     1861 catgtacggc agcaaagcct tcatcaacca cacccagggc atccccgact tctttaagca
     1921 gtccttccct gagggcttca catgggagag aatcaccaca tacgaagacg ggggcgtgct
     1981 gaccgctacc caggacacca gcttccagaa cggctgcatc atctacaacg tcaagatcaa
     2041 cggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacacgcg gctgggaggc
     2101 caacaccgag atgctgtacc ccgctgacgg cggcctgaga ggccacagcc agatggccct
     2161 gaagctcgtg ggcgggggct acctgcactg ctccttcaag accacataca gatccaagaa
     2221 acccgctaag aacctcaaga tgcccggctt ccacttcgtg gaccacagac tggaaagaat
     2281 caaggaggcc gacaaagaga cctacgtcga gcagcacgag atggctgtgg ccaagtactg
     2341 cgacctccct agcaaactgg ggcacagaga tgaatccgga ctcagatctt gagcggccgc
     2401 gactctagat cataatcagc cataccacat tgag
//
