LOCUS       sequence-89195-e        1988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1988)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-H2B-6
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             475..1167
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             1237..1929
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatgagc
      481 gagctgatca aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac
      541 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaag
      601 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc
      661 atgtacggca gcaaagcctt catcaaccac acccagggca tccccgactt ctttaagcag
      721 tccttccctg agggcttcac atgggagaga atcaccacat acgaagacgg gggcgtgctg
      781 accgctaccc aggacaccag cttccagaac ggctgcatca tctacaacgt caagatcaac
      841 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacacgcgg ctgggaggcc
      901 aacaccgaga tgctgtaccc cgctgacggc ggcctgagag gccacagcca gatggccctg
      961 aagctcgtgg gcgggggcta cctgcactgc tccttcaaga ccacatacag atccaagaaa
     1021 cccgctaaga acctcaagat gcccggcttc cacttcgtgg accacagact ggaaagaatc
     1081 aaggaggccg acaaagagac ctacgtcgag cagcacgaga tggctgtggc caagtactgc
     1141 gacctcccta gcaaactggg gcacagaggg catggcaccg gcagcaccgg cagcggcagc
     1201 tccggtaccg cctcctccga ggacaacaac atggccatga gcgagctgat caaggagaac
     1261 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
     1321 gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt ggtcgagggc
     1381 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaagcc
     1441 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
     1501 acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     1561 agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa cttcccatcc
     1621 aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga gatgctgtac
     1681 cccgctgacg gcggcctgag aggccacagc cagatggccc tgaagctcgt gggcgggggc
     1741 tacctgcact gctccttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
     1801 atgcccggct tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag
     1861 acctacgtcg agcagcacga gatggctgtg gccaagtact gcgacctccc tagcaaactg
     1921 gggcacagag atgaatccgg actcagatct tgagcggccg cgactctaga tcataatcag
     1981 ccatacca
//
