LOCUS       sequence-89196-e        3332 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3332)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..3332
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..726
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             796..1488
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatgagcg agctgatcaa ggagaacatg
       61 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag
      121 ggcgaaggca agccctacga gggcacccag accatgaaga tcaaggtggt cgagggcggc
      181 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caaagccttc
      241 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca
      301 tgggagagaa tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
      361 ttccagaacg gctgcatcat ctacaacgtc aagatcaacg gggtgaactt cccatccaac
      421 ggccctgtga tgcagaagaa aacacgcggc tgggaggcca acaccgagat gctgtacccc
      481 gctgacggcg gcctgagagg ccacagccag atggccctga agctcgtggg cgggggctac
      541 ctgcactgct ccttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
      601 cccggcttcc acttcgtgga ccacagactg gaaagaatca aggaggccga caaagagacc
      661 tacgtcgagc agcacgagat ggctgtggcc aagtactgcg acctccctag caaactgggg
      721 cacagagggc atggcaccgg cagcaccggc agcggcagct ccggtaccgc ctcctccgag
      781 gacaacaaca tggccatgag cgagctgatc aaggagaaca tgcacatgaa gctgtacatg
      841 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
      901 gagggcaccc agaccatgaa gatcaaggtg gtcgagggcg gccctctccc cttcgccttc
      961 gacatcctgg ctaccagctt catgtacggc agcaaagcct tcatcaacca cacccagggc
     1021 atccccgact tctttaagca gtccttccct gagggcttca catgggagag aatcaccaca
     1081 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcttccagaa cggctgcatc
     1141 atctacaacg tcaagatcaa cggggtgaac ttcccatcca acggccctgt gatgcagaag
     1201 aaaacacgcg gctgggaggc caacaccgag atgctgtacc ccgctgacgg cggcctgaga
     1261 ggccacagcc agatggccct gaagctcgtg ggcgggggct acctgcactg ctccttcaag
     1321 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggctt ccacttcgtg
     1381 gaccacagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
     1441 atggctgtgg ccaagtactg cgacctccct agcaaactgg ggcacagaga tgaatccgga
     1501 ctcagatctc gagctcaagc ttcgatggcg actgcgaccc ccgtgccgcc gcggatgggc
     1561 agccgcgctg gcggccccac cacgccgctg agccccacgc gcctgtcgcg gctccaggag
     1621 aaggaggagc tgcgcgagct caatgaccgg ctggcggtgt acatcgacaa ggtgcgcagc
     1681 ctggagacgg agaacagcgc gctgcagctg caggtgacgg agcgcgagga ggtgcgcggc
     1741 cgtgagctca ccggcctcaa ggcgctctac gagaccgagc tggccgacgc gcgacgcgcg
     1801 ctcgacgaca cggcccgcga gcgcgccaag ctgcagatcg agctgggcaa gtgcaaggcg
     1861 gaacacgacc agctgctcct caactatgct aagaaggaat ctgatcttaa tggcgcccag
     1921 atcaagcttc gagaatatga agcagcactc aattcgaaag atgcagctct tgctactgca
     1981 cttggtgaca aaaaaagttt agagggagat ttggaggatc tgaaggatca gattgcccag
     2041 ttggaagcct ccttagctgc agccaaaaaa cagttagcag atgaaacttt acttaaagta
     2101 gatttggaga atcgttgtca gagccttact gaggacttgg agtttcgcaa aagcatgtat
     2161 gaagaggaga ttaacgagac cagaaggaag catgaaacgc gcttggtaga ggtggattct
     2221 gggcgtcaaa ttgagtatga gtacaagctg gcgcaagccc ttcatgagat gagagagcaa
     2281 catgatgccc aagtgaggct gtataaggag gagctggagc agacttacca tgccaaactt
     2341 gagaatgcca gactgtcatc agagatgaat acttctactg tcaacagtgc cagggaagaa
     2401 ctgatggaaa gccgcatgag aattgagagc ctttcatccc agctttctaa tctacagaaa
     2461 gagtctagag catgtttgga aaggattcaa gaattagagg acttgcttgc taaagaaaaa
     2521 gacaactctc gtcgcatgct gacagacaaa gagagagaga tggcggaaat aagggatcaa
     2581 atgcagcaac agctgaatga ctatgaacag cttcttgatg taaagttagc cctggacatg
     2641 gaaatcagtg cttacaggaa actcttagaa ggcgaagaag agaggttgaa gctgtctcca
     2701 agcccttctt cccgtgtgac agtatcccga gcatcctcaa gtcgtagtgt acgtacaact
     2761 agaggaaagc ggaagagggt tgatgtggaa gaatcagagg cgagtagtag tgttagcatc
     2821 tctcattccg cctcagccac tggaaatgtt tgcatcgaag aaattgatgt tgatgggaaa
     2881 tttatccgct tgaagaacac ttctgaacag gatcaaccaa tgggaggctg ggagatgatc
     2941 agaaaaattg gagacacatc agtcagttat aaatatacct caagatatgt gctgaaggca
     3001 ggccagactg ttacaatttg ggctgcaaac gctggtgtca cagccagccc cccaactgac
     3061 ctcatctgga agaaccagaa ctcgtggggc actggcgaag atgtgaaggt tatattgaaa
     3121 aattctcagg gagaggaggt tgctcaaaga agtacagtct ttaaaacaac catacctgaa
     3181 gaagaggagg aggaggaaga agcagctgga gtggttgttg aggaagaact tttccaccag
     3241 cagggaaccc caagagcatc caatagaagc tgtgcaatta tgtagggatc caccggatct
     3301 agataactga tcataatcag ccataccaca tt
//
