LOCUS       sequence-89197-e        2846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2846)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(48..110,111..1268)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             1329..2021
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             2091..2783
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag cgccaccatg gcggccccgg
       61 gcgcccggcg tccgctgctc ctgttgctgc tggcaggcct tgcacacagc gccccagcac
      121 tgttcgaggt gaaagacaac aacggcacag cgtgtataat ggccagcttc tctgcctcct
      181 ttctgaccac ctatgaggct ggacatgttt ctaaggtctc gaatatgacc ctgccagcct
      241 ctgcagaagt cctgaagaat agcagctctt gtggtgaaaa gaatgcttct gagcccaccc
      301 tcgcaatcac ctttggagaa ggatatttac tgaaactcac cttcacaaaa aacacaacac
      361 gttacagtgt ccagcacatg tatttcacat ataacctgtc agacacacaa ttctttccca
      421 atgccagctc caaagggccc gacactgtgg attccacaac tgacatcaag gcagacatca
      481 acaaaacata ccgatgtgtc agcgacatca gggtctacat gaagaatgtg accattgtgc
      541 tctgggacgc tactatccag gcctacctgc cgagtagcaa cttcagcaag gaagagacac
      601 gctgcccaca ggatcaacct tccccaacta ctgggccacc cagcccctca ccaccacttg
      661 tgcccacaaa ccccagtgtg tccaagtaca atgtgactgg tgacaatgga acctgcctgc
      721 tggcctctat ggcactgcaa ctcaacatca cctacatgaa gaaggacaac acgactgtga
      781 ccagagcatt caacatcaac ccaagtgaca aatatagtgg gacttgcggt gcccagttgg
      841 tgaccctgaa ggtggggaac aagagcagag tcctggagct gcagtttggg atgaatgcca
      901 cttctagcct gtttttcctg caaggagttc agttgaacat gactcttcct gatgccatag
      961 agcccacgtt cagcacctcc aactattccc tgaaagctct tcaggccagt gtcggcaact
     1021 catacaagtg caacagtgag gagcacatct ttgtcagcaa ggcgctcgcc ctcaatgtct
     1081 tcagcgtgca agtccaggct ttcagggtag aaagtgacag gtttgggtct gtggaagagt
     1141 gtgtacagga cggtaacaac atgctgatcc ccattgctgt gggcggggcc ctggcagggc
     1201 tggtcctcat cgtcctcatc gcctacctca tcggcaggaa gaggagtcac gcgggctatc
     1261 agaccatctc ggaattcggc tccaccggct ccaccggctc caccggcgcg gatccaccgg
     1321 tcgccaccat gagcgagctg atcaaggaga acatgcacat gaagctgtac atggagggca
     1381 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     1441 cccagaccat gaagatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     1501 tggctaccag cttcatgtac ggcagcaaag ccttcatcaa ccacacccag ggcatccccg
     1561 acttctttaa gcagtccttc cctgagggct tcacatggga gagaatcacc acatacgaag
     1621 acgggggcgt gctgaccgct acccaggaca ccagcttcca gaacggctgc atcatctaca
     1681 acgtcaagat caacggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
     1741 gcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg agaggccaca
     1801 gccagatggc cctgaagctc gtgggcgggg gctacctgca ctgctccttc aagaccacat
     1861 acagatccaa gaaacccgct aagaacctca agatgcccgg cttccacttc gtggaccaca
     1921 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gagatggctg
     1981 tggccaagta ctgcgacctc cctagcaaac tggggcacag agggcatggc accggcagca
     2041 ccggcagcgg cagctccggt accgcctcct ccgaggacaa caacatggcc atgagcgagc
     2101 tgatcaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     2161 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgaagatca
     2221 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     2281 acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     2341 tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc gtgctgaccg
     2401 ctacccagga caccagcttc cagaacggct gcatcatcta caacgtcaag atcaacgggg
     2461 tgaacttccc atccaacggc cctgtgatgc agaagaaaac acgcggctgg gaggccaaca
     2521 ccgagatgct gtaccccgct gacggcggcc tgagaggcca cagccagatg gccctgaagc
     2581 tcgtgggcgg gggctacctg cactgctcct tcaagaccac atacagatcc aagaaacccg
     2641 ctaagaacct caagatgccc ggcttccact tcgtggacca cagactggaa agaatcaagg
     2701 aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag tactgcgacc
     2761 tccctagcaa actggggcac agagatgaat ccggactcag atcttgagcg gccgcgactc
     2821 tagatcataa tcagccatac cacatt
//
