LOCUS       sequence-89198-e        1704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1704)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Mito-7
FEATURES             Location/Qualifiers
     source          1..1704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             84..158
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             192..884
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             954..1646
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 catgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
       61 agtgaaccgt cagatccgct agcatgtccg tcctgacgcc gctgctgctg cggggcttga
      121 caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg ggggatccac
      181 cggtcgccac catgagcgag ctgatcaagg agaacatgca catgaagctg tacatggagg
      241 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
      301 gcacccagac catgaagatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
      361 tcctggctac cagcttcatg tacggcagca aagccttcat caaccacacc cagggcatcc
      421 ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagaatc accacatacg
      481 aagacggggg cgtgctgacc gctacccagg acaccagctt ccagaacggc tgcatcatct
      541 acaacgtcaa gatcaacggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa
      601 cacgcggctg ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctgagaggcc
      661 acagccagat ggccctgaag ctcgtgggcg ggggctacct gcactgctcc ttcaagacca
      721 catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcttccac ttcgtggacc
      781 acagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgagatgg
      841 ctgtggccaa gtactgcgac ctccctagca aactggggca cagagggcat ggcaccggca
      901 gcaccggcag cggcagctcc ggtaccgcct cctccgagga caacaacatg gccatgagcg
      961 agctgatcaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc
     1021 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgaaga
     1081 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
     1141 tgtacggcag caaagccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt
     1201 ccttccctga gggcttcaca tgggagagaa tcaccacata cgaagacggg ggcgtgctga
     1261 ccgctaccca ggacaccagc ttccagaacg gctgcatcat ctacaacgtc aagatcaacg
     1321 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacacgcggc tgggaggcca
     1381 acaccgagat gctgtacccc gctgacggcg gcctgagagg ccacagccag atggccctga
     1441 agctcgtggg cgggggctac ctgcactgct ccttcaagac cacatacaga tccaagaaac
     1501 ccgctaagaa cctcaagatg cccggcttcc acttcgtgga ccacagactg gaaagaatca
     1561 aggaggccga caaagagacc tacgtcgagc agcacgagat ggctgtggcc aagtactgcg
     1621 acctccctag caaactgggg cacagagatg aatccggact cagatcttga gcggccgcga
     1681 ctctagatca taatcagcca tacc
//
