LOCUS       sequence-8920-ex       14107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Ventura A, Young AG, Winslow MM, Lintault L, Meissner A, Erkeland 
            SJ, Newman J, Bronson RT, Crowley D, Stone JR, Jaenisch R, Sharp PA,
            Jacks T
  TITLE     Targeted deletion reveals essential and overlapping functions of the
            miR-17 through 92 family of miRNA clusters.
  JOURNAL   Cell. 2008 Mar 7. 132(5):875-86.
  PUBMED    18329372
REFERENCE   2  (bases 1 to 14107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Mar 7. 132(5):875-86."
COMMENT     Sequence Label: pKO-II-miR-17~92
FEATURES             Location/Qualifiers
     source          1..14107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..24
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3264..3297
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4950..4983
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(5014..5061)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5071..5570
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5106..5129)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5503..5522
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5584..6387
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5647..5666)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6257..6276
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(6688..6735)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      11097..11106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        11150..11254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11255..11323,11324..12115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11473..11492)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12286..12874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12775..12794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    12936..13070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12960..12979)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     13014..13033
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     intron          13131..13197
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(13275..13847)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
ORIGIN
        1 cggaccggta aaacgacggc cagtgttaac tctagaattc tggaatttcc tgaaccacag
       61 catgtagcag ggagtgggct tatctcagct ctggggttgt ttacaaggca ctttccgagc
      121 cctgtgctct ctctctacca gagagcccag acttctcggt gctgcactag gcgagggagg
      181 cggctcaggt actggtcgcg aagctccgag cccagcacgc gccccgtcgc acgcggccag
      241 gtttaaggag actttcccgg gaggagctgg acccccaaag gatccgtgat ctacggagat
      301 cggggggggc gacggtgggc gagtggatgt gactcgtctc ccactcgcgt gggcctcccg
      361 atcggcggct acgggagagt gggcgagctg tggtcaccgg gcgcctccgt cgagtccccg
      421 gccagcgcgg ctggcgggaa gcctgagccg cgtgcgctcg caggagggtg cgcggcgcgg
      481 ggtccgcacg cgcggggctg gctcgggagg gcgtgccggg ggcgtggcgc gaggtgtccc
      541 ggcccggagc cctgcgccga gggcagcggg cggcagtggg cggaccccgg gggacgcgcg
      601 gcccggggaa ggcggcgcgg cgccaccagc ggctcccccg gctcccgctc gcctggccgc
      661 caagaacgag ccgccgtggg cggggcctgc ggtgattggc gggcgggcgg ggaggtcgga
      721 agtactttgt tttttatgct aatgagggag tggggcttgt ccgtatttac gttgaggcgg
      781 gagccgccgc ccttcattca cccacatggt ccttcgaggt gccgccgccg ccgccccggc
      841 ccgcgccttc gcgccacttc gcgccctgcg gcgaggccga ggggtgggga cggtccccca
      901 cgccgcccgt gcctctccac ggagccccga gctcggaccg ggccgggcgg gccacgccgc
      961 acccccggcc tggggcctcg ggccggagtg gcgcggaggc tggcgaagat ggtggcggct
     1021 gctgcggtga gtgcgcccgc cccgccgccg gctcagggaa acctgttgtg cgctaagcgg
     1081 agcggcggcg gcggcgggac gggcgggagg gcgggggccg cgggccgggt gggtctcggg
     1141 ccgttgggcg ccggcgtggg gcggccgtcc tggcgcgcac gcgggccgga gggggcccga
     1201 gggggcggcg gcccgggccc cggagaggcc cgcccggcgc acacaatggc cctcgggagg
     1261 cttcgcacga ggccggcggc cgtcccggga gcggcgggag ccgggcgggg cgcgcggccg
     1321 ccgtgcgggg aaagtttctc ccgggggcga gagttaaagc gcctccagaa caaagcggcg
     1381 gcggcacatg gggcaggccg cgggccggga gggggcgcgc ccacgaggaa cctgcgcgcg
     1441 ggcggcgtgg gcgggcggca ctcggcgtcc ccgaaacttt gtgcgcgccg ggccgggctc
     1501 cgggacgcgg gagctgcggg gcgacggggc cggggcgcca ccccgcgctc cgcgtgggct
     1561 ttgtgtgcgg ccgcgtgggc agctcccctc gcgggcgagg cgacccctgc ggtccgcccg
     1621 ggcggcccgg gacgccccgg cccgccctcg gggcggcgcg gagccccgga cgggcgctgg
     1681 ctgcgagcgc ggcgcgggag ggccgggcgg gttcggcagg agcggcggcc ccgccgccat
     1741 gttcctgcgg ggcgggctgc gcgcgccgag ggcggggggg ggggggacat ggcggcgant
     1801 gcgcggcgcc gcgattgttc ccggcttagg cctcgggccg cgtgcggcga gcgccctgtc
     1861 cgcccccctc cctcccccgg agcctggccg gggggcgcgc gggacacaaa ggacggccgg
     1921 cgtgtccgga gcctccccac gctcggccct ccgcgccgcc gccgctcgcc gcgcagccgc
     1981 ccagaaacgg gcgggggttg gggggggggg ggagtctccg nnnccggcgc agcgcggccc
     2041 ggctctgacc tgccgccccc tggcggccgc gcggggaacc cacaagggcc gcctgccccc
     2101 ttgtgcgaca tgtgctgccg gcccgggctc catgagcgtg gcgggtactt ggcaggatcc
     2161 cgtgttcccg tccggttttc tcttgataag ctggcgcgga ggggagaaaa ctggagagcg
     2221 cagcagggct cgtggttctt aggtgaccag agggaagaaa atgagcaacg tgccacgagg
     2281 atcacagcag ttggggaaac aactctttat cccggcgtgc agccacgagg tcctgattgg
     2341 gggaggggtg gtgaagacta gtcctggttg ccctttttct ctagcttgag ctggacagta
     2401 gagttcaggc ttgctgctac tcatcttgca gtatttcagg ggcacattaa ttcgtgcacg
     2461 gatgtgaatc ttggtgggtt ttcaacttta ttttgttaca gcgttttcca cccaatacat
     2521 tgttgccctc aggaccttgt ggtcctggct ctctcttttt tttttttttt tttttttttt
     2581 ttggcaaatg gatagaattg ccccttagga agattaagag aagagcaact tggaatgggg
     2641 ctcggaaagt ggtagtaatt ttgagcaaat gtgcttaccc tttctctgtc ttcaccccac
     2701 ccccaaccct agtcatacac gtggacctag cagcacccga agcattgccc aaggatactt
     2761 gctgagaagg aagtttgcct gagtgggtga gtatattcta gttttgtagc taaaacttct
     2821 tttgagacct ttggttttca cttttgtctt tgagcttgta caacattctt ggtcttttaa
     2881 ggtatgaaat aaatacagtt tgaactcttg tcataaatga gtgagccaca ttttaacgta
     2941 gtaaatctac agtggctttt ggacactaac caaatagttg tagacccttc aagttggagt
     3001 catagagtat ttctaatttg gggtgatact aagacttttt tatgttttat gactaataaa
     3061 cttgaaaatg actaaacaat aatcattaat cttgtcgagt atctgacaat gtggaggaca
     3121 gaagaaaggg attgctgcct ggtcaatgtg aggctttcct ctaaaggtag taccaagtca
     3181 gactgccttc ctgataacag gtctcatttt gtaggaccct tattgtgtgt tttttgggga
     3241 agcctactgt aaaagccaac attataactt cgtataatgt atgctatacg aagttatttt
     3301 aatgggattg tatcttatat ttctttaagg agtttttttt ttttttttaa ggcttacatg
     3361 tgtccaattt ggaacttctg gctattggct cctcccctcc cccccttggg tataagctgt
     3421 aattgatgtt tgtgacagaa tttagagctt tggctttttc ctttttgtct aattatttat
     3481 ttcaaattta gcaggaataa agtgaacctc accttgggac tgaagctgtg accagtcaga
     3541 ataatgtcaa agtgcttaca gtgcaggtag tgatgtgtgc atctactgca gtgagggcac
     3601 ttgtagcatt atgctgacag ctgcctcggt gggagccaca gtgggcgctg cctcgggcgg
     3661 cactggctgc gtccagtcgt cggtcagtcg gtcgcgggga gggcctgctg gtgctgcgtg
     3721 ctttttgttc taaggtgcat ctagtgcaga tagtgaagta gactagcatc tactgcccta
     3781 agtgctcctt ctggcataag aagttatgtc ctcatccaat ccaagtcaag caagcatgta
     3841 ggggtctctc catagttgtg tttgcagccc tctgttagtt ttgcatagtt gcactacaag
     3901 aagaatgtag ttgtgcaaat ctatgcaaaa ctgatggtgg cctgctattt acttcaagtg
     3961 ttgttttttt ttaaactaat tttgtatttt tattgtgtcg atgtagagcc tgcgtggtgt
     4021 gtgtgatgtg acagcttctg tagcactaaa gtgcttatag tgcaggtagt gtgtagccat
     4081 ctactgcatt acgagcactt aaagtactgc cagctgtaga actccagcct cgcctggcca
     4141 tcgcccagcc aactgtcctg ttattgagca ctggtctatg gttagttttg caggtttgca
     4201 tccagctgta taatattctg ctgtgcaaat ccatgcaaaa ctgactgtgg tggtgaaaag
     4261 tctgtagaga agtaagggaa aatcaaaccc ctttctacac aggttgggat ttgtcgcaat
     4321 gctgtgtttc tctgtatggt attgcacttg tcccggcctg ttgagtttgg tggggattgt
     4381 gaccagaaga tgtgaaaatg acaatattgc tgaagatgcc gatttccact gtaaaaatgt
     4441 tcacgagctg atggagtgta tgaacacatt ctgtaaactg tgatttctat tgctttcgtt
     4501 ggtgtgtaaa acgggatttt tttaattgaa atttgtgttt ttaaattgtg gtttgttttt
     4561 actgaatcat ttcattttcg tgtttgctcg ctggatttag taagaagttg tgtgtggggg
     4621 cttaggtgaa gttaaactgc cttttgaacc tggtatgact tcaggtttat tttactctaa
     4681 aacccattaa catgagttaa gaaaaatgtt actttctttg gatctctgtt tattaaagtg
     4741 tgacaattca gatggagtgt tttctgataa gattattcct aatccgtgag ttcttaacag
     4801 ttaacaggtg gtggccactc tgttaatgtg cacaccctgt aagaaagaac tatccttgga
     4861 ctgttcttac catgtgaccc cctcaaggga aagatggcaa actgatggtc agtagagtga
     4921 caggtacaca tgacactcga gggcgcgcca taacttcgta taatgtatgc tatacgaagt
     4981 tattaggtac tgccgggcct cttgagggga attgaagttc ctatactttc tagagaatag
     5041 gaacttcgga ataggaactt caattctacc gggtagggga ggcgcttttc ccaaggcagt
     5101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     5161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     5221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     5281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     5341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     5401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     5461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     5521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     5581 aatatgggat cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5641 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5701 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5761 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5821 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5881 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5941 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6001 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6061 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6121 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     6181 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     6241 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6301 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6361 tatcgccttc ttgacgagtt cttctgaggg gatccgctgt aagtctgcag aaattgatga
     6421 tctattaaac aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag
     6481 aagggtgaga acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg
     6541 tggggtggga ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga
     6601 taatgtttca tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc
     6661 attcctccca ctcatgatct atagatcgaa gttcctatac tttctagaga ataggaactt
     6721 cggaatagga acttcggcgc gccgggatcc ctagaccgcg gctagaatcg atcctagaaa
     6781 gcttcctaga cccggggggt cgagtgctgg gtctggagaa gctgcagtta gtatttagga
     6841 taacagtata tataatatat agttctacat ttgggcagca cagttggttt caggctatga
     6901 ataaaaatca ttggagtgaa acctaaagaa aagtaaaatt aataaggaag agctgctaaa
     6961 atcaggttta agctgacact atctacagag ctaaagtttt ccataatatg ttgctttttt
     7021 taaaagaaat gcaagaagct gactgatgga tccctgagct gccaggtaag gattccacag
     7081 gcctgggttt gattgtgagt gaccagaatt gtacaattat tactaaaaca gaaacatagt
     7141 cacttctgac tccattctta acatttttgg attaaaactt gtttcagatg actaatgaaa
     7201 acttccttta aaacgtgata aagctctaat gtcagcacga tccacatgat ccatgtgcct
     7261 cctataaagg aggggtctgt caccagtgtt acggattgaa tgctacgttt atcttccaac
     7321 ataggaagcc tgccaggtac tttcttcaat atactaaatt agaagtttaa aattaataag
     7381 gtcttaatta ggacctttgg atgctgagct tgtggtagta attgtgttca gaatgttttg
     7441 aacttaaatt gagatttaat tttagtaagg aatgtgtgtc ttagaggcct agtagtgaag
     7501 aggagttttc ctaggatttt cctctctcct cccttaatta agctgctttc tgcactaagg
     7561 gcttcacgca gcaacgcact tgttcagttc cgcacagggg gctttgctga ctgtctgctc
     7621 taaaactctg ttggcaaaac agctgtggtc tctcttagac tttgattttg tagtaactag
     7681 ggcatatagt tgctataagc tttcaggaat gggggtgggt tactaatgtc ttatgttata
     7741 tgaaagtagt taaatttatc ctatattaag aaggaagcat tattgagact atatactgtt
     7801 gattttacta aaaactctaa gatgtccatt ttaacaggca aacctagcac agaaaaccag
     7861 ctggattttc cctgtgcatg gtttgaagag tcagtcctac ataattgctg acacaatgca
     7921 acctgcaact gcccggagaa agaacagttc actaaaattt gttgtattta tcatccaatt
     7981 ctgttctgta actggtaaca ctagtttgtc tggctttaga gaataggtga atctctaaaa
     8041 cagtagaaac agctcagttg ggcaagggcc gttctagtag catgcctgct ccttggagtt
     8101 ttccagatta ttttgtatag cctattccag attcttgtct aagattgctt tgctctgtgc
     8161 actcagaatt ggtgtgcctt tctatttgtg taaatgatat ataatacatt gctagttgtc
     8221 taggatctat tcaggaagtg cgtgctgtga attttaaagt atgggaagat tgttaacaag
     8281 ggtttcaatg ttttgttttt ttttttctgg taagctaaaa tagaacattg tgaggtacct
     8341 ggtttattgt ggttacagac ttggaataat gttcattgtt ttgtgctaat aaattgtgtt
     8401 aaaatttggt gtacatatga gcatttttaa ataaagttat tttgtagcac tgatgatctt
     8461 gtatcttgtt ttttcctgta agtggcccaa atatcttttt cttgttgagg gcatggggag
     8521 accagtctta gctttaacca tggagtttgt cttttgcctt aatttacagg gaagaggaaa
     8581 tcattgtgtg tcctgatttc aaacctcaga ataaggaaaa ttagcagggt accttttaca
     8641 agttggtcag aaagcaacaa attgtgcttg tgtgtttgac ttgggttctt aggattttgt
     8701 aggaaagtct ccaggatcaa atgctaagat gatggacgac acaaagccta tctgcccact
     8761 caggactaga acactgtatg tatgctgcag ttaagacttg gagactgctc gggggaatga
     8821 gaggtctaaa atggttatga caactgatga agcagtgtat gacgctcaag gattactata
     8881 tagcaaagat cttaagtcat gacagtaaag aagggctact aaatgccagt gacttaagaa
     8941 ttgaaggctc atggagtgct gaaagtgctc atgtagcatt gggaaagtat gtaggtgcaa
     9001 catgagtgca gaatttgttc ttagtaagta gtgagcatga gtctttaata caagttgtag
     9061 agctgtgtga gaccctgtac caatgtttct tccaaattga gactggtcat tagaatttaa
     9121 ttctatggat ggagttaggc tatagcagga aggagctagg ccttgcaatt tgagttctct
     9181 aagacttttg atagcagtct gctctacttc tgttcttgac catgatgcca aggtgggtac
     9241 catgaaagta ctgtgcgtga ttctaggctc ctgcgttcta atatagtagc accatttgta
     9301 tttgtgaaaa ggatgattgg cagtgttttc aggactcttg gtttttatct atgcataggt
     9361 gttaagtaga actattggcc tgagttgtcc aaagtcatgc cttcaatgct taggaacccc
     9421 aaggcccggt cttttaatag gttatacttt gcagagtcca tatgcattca ctttctctta
     9481 ggcttatact tgaagctagc tgcaagatag aattcgttgt gtatgcagca caaccttttt
     9541 taaaaaccac aactattggg ttttttgctc agttgcacac ttaattgatt cctgttctac
     9601 cactcttgac cccatacttg aagtctgcct tggattcttg tgtgggagct ggggttctaa
     9661 ggtgcaggct agtactttaa aattgtgcta attctttgat actttgtatc ttattttgac
     9721 cacagcagtg tgcatatact gtctgcctct tggctccctg cctttcacat tttcaggtag
     9781 tcttataact taccagtttt tctgggcata tgtatattag aagaggatct tcagtaagga
     9841 aggccttttt caaaaactcc taccacctga gatgtgagaa gtgaatctgg agtacattcc
     9901 caagcctcat ttgatggtca tttttaaaag ttgggtgtgc ctagtagctc tgtgtgtaat
     9961 agaacaaggc aggccttcag caagttagca aaatgccctg tattagtgct gctagaagga
    10021 aaagggcatg ggcggttgat gcacatagtg gagcagaagc aaattgaggt ggtggataca
    10081 tctctgcttc gggtagtgag gaattctgtt agattggaat ataactgaca gcctctcact
    10141 aaaagtgagt taaagttaac tacgggtgag atgatggaaa tggtccttca aaattagagc
    10201 tgagtctcca ggactgaaac ctaagaccct tagctggcct gagcttactg ggtgctctgt
    10261 ggaagattat ttcacattgg ttagatttgt caatgctaac tagaactaca aatatttcta
    10321 cagaggatct ccccacaaac agacctggaa cacaggggtt acattttttc tgtgccttga
    10381 gttcttagca cctagtagca aagcaataca gtaatttcgg tggtatggtg gtatttgagt
    10441 taggttgttt gtggtttaag aggaagcagc atttggttat cttcgaatac aaaattgtgg
    10501 ccagtgattc tcttagagca atgaacacct ctgtagttat tctagtgtta ggtttgtaat
    10561 gtgtcagctc caatttggag actgctcttc caagttactt ggctaataga ttagtattag
    10621 ctgggtatga tggcacatgc ctttaaaccc agcacttggg aggcagaggc aggtggattt
    10681 ctgagttcca ggccagagtt ccaggacagc tagggttaca cagaaaccct gtctcgaacc
    10741 ccgcccccaa aggggggtgg tggtgtttct ctcattattt aacaagtatg ttttcccaga
    10801 attaatgaga actagtgtcc agttcaccaa gaggtaccag gaatatccaa atctcgggcc
    10861 tatgccttat aaattatttc tggtgctact gaataaggaa agctacttgg gctgaggcac
    10921 agatactgtg aaaacgttac ttgcctagtt aaaacattag aagacagacc tttgtgggct
    10981 gcagttgtcc ttattgaggg ttgatgtgag gaggaaggat ataggtaggt agtgacttgt
    11041 gagtgcagct gagcaagctt gatatcgaat tcctgcagcc cgggggggtc gacgcggccg
    11101 ccatggtcat agctgtttga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    11161 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    11221 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    11281 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    11341 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    11401 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    11461 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    11521 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    11581 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    11641 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    11701 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    11761 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    11821 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    11881 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    11941 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    12001 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    12061 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    12121 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    12181 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    12241 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    12301 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    12361 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    12421 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    12481 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12541 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12601 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12661 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12721 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12781 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12841 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    12901 gttcctggcc ttttgccggt ccgctctaga actacgatcc agacatgata agatacattg
    12961 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
    13021 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt agatcctagt
    13081 ggatctgcat tccaccactg ctcccattca tcagttccat aggttggaat ctaaaataca
    13141 caaacaatta ggaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt
    13201 agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga agtaaggttt
    13261 ccttcacaaa gagatcgcct gacacgattt cctgcacagg cttgagccat atactcatac
    13321 atcgcatctt ggccacgttt tccacgggtt tcaaaattaa tctcaagttc tacgcttaac
    13381 gctttcgcct gttcccagtt attaatatat tcaacgctag aactcccctc agcgaaggga
    13441 aggctgagca ctacacgcga agcaccatca ccgaaccttt tgataaactc ttccgttccg
    13501 acttgctcca tcaacggttc agtgagactt aaacctaact ctttcttaat agtttcggca
    13561 ttatccactt ttagtgcgag aaccttcgtc agtcctggat acgtcacttt gaccacgcct
    13621 ccagcttttc cagagagcgg gttttcatta tctacagagt atcccgcagc gtcgtattta
    13681 ttgtcggtac tataaaaccc tttccaatca tcgtcataat ttccttgtgt accagatttt
    13741 ggcttttgta tacctttttg aatggaatct acataaccag gtttagtccc gtggtacgaa
    13801 gaaaagtttt ccatcacaaa agatttagaa gaatcaacaa catcatcagg gtccatggcg
    13861 aggacctgca ggtcgcagat cctgaacggc agaggttacg gcagtttgtc tctccccctt
    13921 ccgggagcca ccttcttctc caaccgtccc ggtcgcgctc tcggcgcttc tgaggagaga
    13981 actggctgag tgacgccctt tatagattcg cccttgtgtc ccgcccttcc tttcccgccc
    14041 tcccttgcgc tacggggccg cccgcaccgg cctacacgga gcgcgcgcgg cggagttgtt
    14101 cggtccg
//
