LOCUS       sequence-89204-e        3289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3289)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..3289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1772..2464
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             2534..3226
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 taccggactc agatctcgag ctcaagcttc gaattccatg gcggcccccc gcccgtctcc
       61 cgcgatcccc gtttcggtct cggctccggc tttttacgcc ccgcagaaga agttcggccc
      121 tgtggtggcc ccaaagccca aagtgaatcc cttccggccc agggacagcg agcctccccc
      181 ggcacccggg gcccagcgcg cacagatggg ccgggtgggc gatattcccc cgccgccccc
      241 ggaagacttt cccctgcctc cacctcccct tgctggggat ggcgacgatg cagagggtgc
      301 tctgggaggt gccttcccgc cgccccctcc cccgatcgag gaatcatttc cccctgcgcc
      361 tctggaggag gagatcttcc cttccccgcc gcctcctccg gaggaggagg gagggcctga
      421 ggcccccata ccgcccccac cacagcccag ggagaaggtg agcagtattg atttggagat
      481 cgactctctg tcctcactgc tggatgacat gaccaagaat gatcctttca aagcccgggt
      541 gtcatctgga tatgtgcccc caccagtggc cactccattc agttccaagt ccagtaccaa
      601 gcctgcagcc gggggcacag cacccctgcc tccttggaag tccccttcca gctcccagcc
      661 tctgccccag gttccggctc cggctcagag ccagacacag ttccatgttc agccccagcc
      721 ccagcccaag cctcaggtcc aactccatgt ccagtcccag acccagcctg tgtctttggc
      781 taacacccag ccccgagggc ccccagcctc atctccggct ccagccccta agttttctcc
      841 agtgactcct aagtttactc ctgtggcttc caagttcagt cctggagccc caggtggatc
      901 tgggtcacaa ccaaatcaaa aattggggca ccccgaagct ctttctgctg gcacaggctc
      961 ccctcaacct cccagcttca cctatgccca gcagagggag aagccccgag tgcaggagaa
     1021 gcagcacccc gtgcccccac cggctcagaa ccaaaaccag gtgcgctccc ctggggcccc
     1081 agggcccctg actctgaagg aggtggagga gctggagcag ctgacccagc agctaatgca
     1141 ggacatggag catcctcaga ggcagaatgt ggctgtcaac gaactctgcg gccgatgcca
     1201 tcaacccctg gcccgggcgc agccagccgt ccgcgctcta gggcagctgt tccacatcgc
     1261 ctgcttcacc tgccaccagt gtgcgcagca gctccagggc cagcagttct acagtctgga
     1321 gggggcgccg tactgcgagg gctgttacac tgacaccctg gagaagtgta acacctgcgg
     1381 ggagcccatc actgaccgca tgctgagggc cacgggcaag gcctatcacc cgcactgctt
     1441 cacctgtgtg gtctgcgccc gccccctgga gggcacctcc ttcatcgtgg accaggccaa
     1501 ccggccccac tgtgtccccg actaccacaa gcagtacgcc ccgaggtgct ccgtctgctc
     1561 tgagcccatc atgcctgagc ctggccgaga tgagactgtg cgagtggtcg ccctggacaa
     1621 gaacttccac atgaagtgtt acaagtgtga ggactgcggg aagcccctgt cgattgaggc
     1681 agatgacaat ggctgcttcc ccctggacgg tcacgtgctc tgtcggaagt gccacactgc
     1741 tagagcccag acggatccac cggtcgccac catgagcgag ctgatcaagg agaacatgca
     1801 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg
     1861 cgaaggcaag ccctacgagg gcacccagac catgaagatc aaggtggtcg agggcggccc
     1921 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca aagccttcat
     1981 caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg
     2041 ggagagaatc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagctt
     2101 ccagaacggc tgcatcatct acaacgtcaa gatcaacggg gtgaacttcc catccaacgg
     2161 ccctgtgatg cagaagaaaa cacgcggctg ggaggccaac accgagatgc tgtaccccgc
     2221 tgacggcggc ctgagaggcc acagccagat ggccctgaag ctcgtgggcg ggggctacct
     2281 gcactgctcc ttcaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc
     2341 cggcttccac ttcgtggacc acagactgga aagaatcaag gaggccgaca aagagaccta
     2401 cgtcgagcag cacgagatgg ctgtggccaa gtactgcgac ctccctagca aactggggca
     2461 cagagggcat ggcaccggca gcaccggcag cggcagctcc ggtaccgcct cctccgagga
     2521 caacaacatg gccatgagcg agctgatcaa ggagaacatg cacatgaagc tgtacatgga
     2581 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
     2641 gggcacccag accatgaaga tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     2701 catcctggct accagcttca tgtacggcag caaagccttc atcaaccaca cccagggcat
     2761 ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagaa tcaccacata
     2821 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ttccagaacg gctgcatcat
     2881 ctacaacgtc aagatcaacg gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     2941 aacacgcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctgagagg
     3001 ccacagccag atggccctga agctcgtggg cgggggctac ctgcactgct ccttcaagac
     3061 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcttcc acttcgtgga
     3121 ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgagat
     3181 ggctgtggcc aagtactgcg acctccctag caaactgggg cacagagatg aatccggact
     3241 cagatcttga gcggccgcga ctctagatca taatcagcca taccacatt
//
