LOCUS       sequence-89208-e        3932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3932)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..3932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..1472
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     regulatory      1527..1536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1578..1613
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
       61 aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
      121 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      181 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
      241 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
      301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
      361 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
      421 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      481 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
      541 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
      601 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
      661 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
      721 tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca
      781 acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga
      841 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      901 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
      961 cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca
     1021 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1081 gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
     1141 agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     1201 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
     1261 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
     1321 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
     1381 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
     1441 tgttcctgta cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt
     1501 cgaattctgc agtcgacggt accgcggcca ccatggctac tcaagctgac ctgatggagt
     1561 tggacatggc catggagccg gacagaaaag ctgctgtcag ccactggcag cagcagtctt
     1621 acttggattc tggaatccat tctggtgcca ccaccacagc tccttccctg agtggcaagg
     1681 gcaaccctga ggaagaagat gttgacacct cccaagtcct ttatgaatgg gagcaaggct
     1741 tttcccagtc cttcacgcaa gagcaagtag ctgatattga cgggcagtat gcaatgacta
     1801 gggctcagag ggtccgagct gccatgttcc ctgagacgct agatgagggc atgcagatcc
     1861 catccacgca gtttgacgct gctcatccca ctaatgtcca gcgcttggct gaaccatcac
     1921 agatgttgaa acatgcagtt gtcaatttga ttaactatca ggatgacgcg gaacttgcca
     1981 cacgtgcaat tcctgagctg acaaaactgc taaacgatga ggaccaggtg gtagttaata
     2041 aagctgctgt tatggtccat cagctttcca aaaaggaagc ttccagacat gccatcatgc
     2101 gctcccctca gatggtgtct gccattgtac gcaccatgca gaatacaaat gatgtagaga
     2161 cagctcgttg tactgctggg actctgcaca acctttctca ccaccgcgag ggcttgctgg
     2221 ccatctttaa gtctggtggc atcccagcgc tggtgaaaat gcttgggtca ccagtggatt
     2281 ctgtactgtt ctacgccatc acgacactgc ataatctcct gctccatcag gaaggagcta
     2341 aaatggcagt gcgcctagct ggtggactgc agaaaatggt tgctttgctc aacaaaacaa
     2401 acgtgaaatt cttggctatt acaacagact gccttcagat cttagcttat ggcaatcaag
     2461 agagcaagct catcattctg gccagtggtg gaccccaagc cttagtaaac ataatgagga
     2521 cctacactta tgagaagctt ctgtggacca caagcagagt gctgaaagtg ctgtctgtct
     2581 gctctagcaa caagccggcc attgtagaag ctggtgggat gcaggcactg gggcttcatc
     2641 tgacagaccc aagtcagcga cttgttcaaa actgtctttg gactctcaga aacctttcag
     2701 atgcagcgac taagcaggaa gggatggaag gcctccttgg gactctagtg cagcttctgg
     2761 gttccgatga tataaatgtg gtcacctgtg cagctggaat tctctctaac ctcacttgca
     2821 ataattacaa aaacaagatg atggtgtgcc aagtgggtgg catagaggct cttgtacgca
     2881 ccgtccttcg tgctggtgac agggaagaca tcactgagcc tgccatctgt gctcttcgtc
     2941 atctgaccag ccggcatcag gaagccgaga tggcccagaa tgccgttcgc cttcattatg
     3001 gactgcctgt tgtggttaaa ctcctgcacc caccatccca ctggcctctg ataaaggcaa
     3061 ctgttggatt gattcgaaac cttgcccttt gcccagcaaa tcatgcgcct ttgcgggaac
     3121 agggtgctat tccacgacta gttcagctgc ttgtacgagc acatcaggac acccaacggc
     3181 gcacctccat gggtggaacg cagcagcagt ttgtggaggg cgtgcgcatg gaggagatag
     3241 tagaagggtg tactggagct ctccacatcc ttgctcggga cgttcacaac cggattgtaa
     3301 tccgaggact caataccatt ccattgtttg tgcagttgct ttattctccc attgaaaata
     3361 tccaaagagt agctgcaggg gtcctctgtg aacttgctca ggacaaggag gctgcagagg
     3421 ccattgaagc tgagggagcc acagctcccc tgacagagtt actccactcc aggaatgaag
     3481 gcgtggcaac atacgcagct gctgtcctat tccgaatgtc tgaggacaag ccacaggatt
     3541 acaagaagcg gctttcagtc gagctgacca gttccctctt caggacagag ccaatggctt
     3601 ggaatgagac tgcagatctt ggactggaca ttggtgccca gggagaagcc cttggatatc
     3661 gccaggatga tcccagctac cgttcttttc actctggtgg atacggccag gatgccttgg
     3721 ggatggaccc tatgatggag catgagatgg gtggccacca ccctggtgct gactatccag
     3781 ttgatgggct gcctgatctg ggacacgccc aggacctcat ggatgggctg cccccaggtg
     3841 atagcaatca gctggcctgg tttgatactg acctgtaaaa gcttggatcc accggatcta
     3901 gataactgat cataatcagc cataccacat tt
//
