LOCUS       sequence-89209-e        4270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 554, 
            Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4270)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..4270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..1460
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    3279..3290
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggtcatca
       61 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
      121 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      181 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
      241 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
      301 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
      361 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
      421 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
      481 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
      541 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
      601 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
      661 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca
      721 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
      781 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
      841 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      901 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
      961 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     1021 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     1081 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     1141 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     1201 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     1261 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     1321 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     1381 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     1441 gcatggacga gctgtacaag tccggactca gatctggcag cggtggaggc ttggaggagc
     1501 cggaggccgc gacgcggaca gccgcggccg tggattgcaa agacagaccc gggttcccag
     1561 tgaagaggtt aatacaagcc cgcttgccct tcaaacgcct gaaccttgtt cccaaggaga
     1621 aagttgagga agacacatca ccaaaagcag ctgtggaaag caaagtcccg gatctacaat
     1681 tgtctttggg cacttttgaa agccagtgtc acacaggctc tcacgtaggc ttgagtacaa
     1741 agttggtcgg tggccagggc cccattgata gcttcttgag ggcaacaatc aagccagtac
     1801 cgagtgtggt cattatcgac ctgacggaga actgcagtga cattccagac agccctgagg
     1861 gccacagtga actaagtcct gacactgctg gtgtggtcac cacagtggaa ggagctgcca
     1921 agcagcagga gcactctgca gcagagctgt gtctcctaga gaccccttca gacatcactt
     1981 gtcacatgga agaggagcct gggagtccag gagacccaaa gaggacgggt gactgtcagg
     2041 ctggttcact acagagctgt ccggagctga ccccaggttc taggacatgc cccaccaagg
     2101 agttatcaag ctggagcaag gcaggggatc tcctatttat agagaaagtg cctgtggtgg
     2161 tgctagagga catcctggcc actaagccct ccattgcctc tcttcccatg atgtccctgg
     2221 ataggagtgt cacttcagag agtgaaatac tggagtcctg ccccgaggat gactccatac
     2281 tgagccactc ctccaccaac tcctcttctc caaccagctc tcctgaggga ccatccacac
     2341 ccccagaaca tcggggtggg agaagctccc cctccacccc agcctgcaga gttgccaaga
     2401 actttgtcaa aggctccaca gagaagggca ggagcaaact gcacagagat agagaacagc
     2461 agagggaaga gaaggaaaag ctgagggaag agatacgacg agccaaggag gaggcacgga
     2521 agaagaagga ggaggagaag gagctgaagg aaaaggagcg gagggaaaag cgggagaaag
     2581 atgagaagga gaaggcggag aagcagcggc taaaggagga aaaacgcaag gagcggcagg
     2641 aagctctgga ggctaaactg gaggagaaga ggaagaagga ggaagagaag cggttacggg
     2701 aggaagagaa gcggttacgg gaagaagaga agcgcattaa ggcagaaaag gcagagatca
     2761 ctcgattctt ccagaaaccg aagactccgc aggctcccaa gaccctggcc ggttcctgtg
     2821 gtaagtttgc cccctttgaa atcaaagaac acatggtgct ggctccacgg tgccgagctg
     2881 ccttggatca agacctgtgc gatcagctgg accagctcct gcagcagcag agtgtggcaa
     2941 gcaccttcct cagtgaccta aaaagccggc tgcccttgag atcggggccc acccgggtct
     3001 gtggccatga cacagacatc atgaacaggg atgtggtcat tgtggagagc agcaaggtgg
     3061 acggtgtgtc tgaaaggaag aagtttggcc gcatgaagct tctgcagttc tcagagaacc
     3121 accggccagc atactggggg acgtggaaca agaagacagc catcatccgc ccccggaacc
     3181 cctgggccca ggacaaagac cttcttgact atgaggtaga cagtgacgat gagtgggaag
     3241 aggaggaacc tggggagtct ttgtcccaca gtgaggggga tgaggatgac gatgtggggg
     3301 aagatgagga tgaggacgat ggcttctttg tgccccatgg gtacttatct gaagatgaag
     3361 gcgtgactga ggagtgtgca gaccctgaga accacaaagt acaccagaag ctgaaggcca
     3421 aggagtggga tgagctccta gccaagggca agcgctttcg tgtgctgcag cctgtgcacg
     3481 tgggatgtgt atgggcatca gaggcagcca actgcacgag ttccgacttg aagctgctgc
     3541 agcagttcac tgcatgcctg ctggatgtgg catcccctga tgagccagaa cccggggcct
     3601 ccaggaggga aaagagggac cagcatatcc tggcccagtt gcttccgctg ctacatggca
     3661 atgtgaatgg gagcaaggtg atcatccatg aattccagga gcaatgccgc aggggcctgc
     3721 tgacccttcc aagtcccacc ccacacctgc agatgcccaa cttggaggat gctgttgctg
     3781 tgccatccaa ggccaggctg aagcgcctta tctctgagaa ctctgcctat gagaagcggc
     3841 ccaacttccg tatgtgctgg tatgtgcacc cagaggtgct caagagcttt ggccaggagt
     3901 gcctgccagt gccctgccag tggacctaca tcaccaccat gccctcagca cccagggaag
     3961 acagtggcag tgcttctact gaggggcctg gccagagcac acctatgcta ctcaagcgga
     4021 agccagcggc caccatgtgc atcacgcagt ttatgaagaa gcggcgctat gatggacagg
     4081 ttgggtctgg ggacatggat ggcttccagg cagacacaga agaggatgaa gaggatgaca
     4141 cagactgtat gatcatagat gtgccagatg ttgggagtga cgtgtcagaa gctcccatac
     4201 ctgctcccac actttgcaag tgaggatcca ccggatctag ataactgatc ataatcagcc
     4261 ataccacatt
//
