LOCUS       sequence-8921-ex       11917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Ventura A, Young AG, Winslow MM, Lintault L, Meissner A, Erkeland 
            SJ, Newman J, Bronson RT, Crowley D, Stone JR, Jaenisch R, Sharp PA,
            Jacks T
  TITLE     Targeted deletion reveals essential and overlapping functions of the
            miR-17 through 92 family of miRNA clusters.
  JOURNAL   Cell. 2008 Mar 7. 132(5):875-86.
  PUBMED    18329372
REFERENCE   2  (bases 1 to 11917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Mar 7. 132(5):875-86."
COMMENT     Sequence Label: pKS-DTA miR-106b~25
FEATURES             Location/Qualifiers
     source          1..11917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    785..1009
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          1083..1148
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1225..1794)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphteria toxin Fragment A"
                     /label=DTA
                     /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
                     DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
                     LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
                     FETRGKRGQDAMYEYMAQACAGNRVRR"
     promoter        complement(1821..2314)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    5357..5390
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        5454..5953
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        5965..6012
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6031..6834
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6094..6113)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6704..6723
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6872..7096
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7151..7184
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(9729..9749)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9729..9747)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9768..9784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9768..9784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9781..9803)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9792..9808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9816..9822,9823..9840,9841..9846))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9861..9882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9999..10016)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10170..10758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10250..10269)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10929..11720,11721..11789))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11552..11571
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11790..11894)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaggccgcaa tggccttaat taagtttaaa cggccgactt
      721 ggccggccgg cctctagatg catgctcgag gtcgagcccc agctggttct ttccgcctca
      781 gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
      841 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
      901 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
      961 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
     1021 cagcgagctc taggatctgc attccaccac tgctcccatt catcagttcc ataggttgga
     1081 atctaaaata cacaaacaat tagaatcagt agtttaacac attatacact taaaaatttt
     1141 atatttacct tagagcttta aatctctgta ggtagtttgt ccaattatgt cacaccacag
     1201 aagtaaggtt ccttcacaaa gagatcgcct gacacgattt cctgcacagg cttgagccat
     1261 atactcatac atcgcatctt ggccacgttt tccacgggtt tcaaaattaa tctcaagttc
     1321 tacgcttaac gctttcgcct gttcccagtt attaatatat tcaacgctag aactcccctc
     1381 agcgaaggga aggctgagca ctacacgcga agcaccatca ccgaaccttt tgataaactc
     1441 ttccgttccg acttgctcca tcaacggttc agtgagactt aaacctaact ctttcttaat
     1501 agtttcggca ttatccactt ttagtgcgag aaccttcgtc agtcctggat acgtcacttt
     1561 gaccacgcct ccagcttttc cagagagcgg gttttcatta tctacagagt atcccgcagc
     1621 gtcgtattta ttgtcggtac tataaaaccc tttccaatca tcgtcataat ttccttgtgt
     1681 accagatttt ggcttttgta tacctttttg aatggaatct acataaccag gtttagtccc
     1741 gtggtacgaa gaaaagtttt ccatacaaaa gattagaaga ataacaacat catcaggatc
     1801 catggcgagg acctgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca
     1861 gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc
     1921 ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc
     1981 cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat
     2041 tgctcagcgg tgctgtccat ctgacgagac tagtgagacg tgctacttcc atttgtcacg
     2101 tcctnacgac gcgagctgcg gggcgggggg gaacttcctg actaggggag gagnagaagg
     2161 tggcgcgaag gggccaccaa agaacggagc cggttggcgc ctaccggtgg atgtggaatg
     2221 tgtgcgaggc canaggccac ttgtgtagcg ccaagtgcca gcggggctgc taagcgcatg
     2281 ctcagactgc cttggaaaag cgcctcccct acccggtaga attcgatatc aagcttaaga
     2341 gcatgtgagt ttaggggata tgagtcctgc agctggttat gaaattatag gaagaaagat
     2401 tgggagagag gtttacctag aattttttta tatttgctct tagtcagaaa atcgggaggt
     2461 gatttaattg aagttttttt ctggttctat tgggaggagg ataagaagaa ctcagcttcc
     2521 ttcctgaagt catctaggtt gggatgactg aatcttacca tcaaatgggc atcttctggg
     2581 gtggtggtca gttgggtcag gtctgcctcg cttttctcct tgtctcatgt gctctgtgtc
     2641 atgccctccc ccacacctcc caatctccta gagtgaccaa gttcctgtgg gaaacatccc
     2701 tcgtagcatc acagtggttc tagaaggtga gaacacaaga attgcccagc ctggtgacca
     2761 tgtcagcgtc actggcatct tcttgccagt cctgcgcaca gggttccaac agatggcaca
     2821 ggtgagggcg ttttgaatga ggaaatagct gccttgcctc ctcatccacc cactcactct
     2881 tcacaccctt agaaaatgaa aatcagtgag gtggaacttg tggataaaaa attctcaggg
     2941 actctggtga tggggacttt aaagggaact cttgtgtcca gggcttactc tcagaaactt
     3001 acctggaagc ccactggatt gtgaagatga caaagagtga cgatgatgtc tctggggccg
     3061 gagagctgag ctcagaggag ctgaagcaga ttgcaggtga gggaccacga gctcaagttg
     3121 agagttgagc tccatcctag attttgtcct gaaggaaatg ctatggctta attttttgca
     3181 gaggaggatt tctatgagaa gctggccgct tctattgctc cggagatcta tggacatgaa
     3241 gatgtgaaga aggcgctgct gctgcttctg gtgggtggtg tggaccagtc tcctcaaggc
     3301 atgaagatca gaggtaagaa ggacaagaga cttaggaggg agggtaaaaa aacatcccac
     3361 gagagtctgt aaatgacaag agttgatgat ttaccttagt ggttgttgtg tcttagaagt
     3421 agtctggact ttaagaaggg accaggtctc aagtgctctt ctctgttggt ggacaacttg
     3481 ggtaaagagt atcactcagg cttccttctc atttgccagg aaacatccac atctgcctta
     3541 tgggagaccc tggtgtggcc aaatctcagc tcctatctta catcgaccgt ctggcaccgc
     3601 gaagtgagtg tgaacaggga tgaggcttgg tgtttggttg ataaacacta aggcctctgg
     3661 aaggcctggg gaaagcggtt cctttcttca gcagtttgct ctttatcttt gcaggtcagt
     3721 acacaacagg ccgtggctcc tctggtgtag ggctcacagc agctgtgttg agagactctg
     3781 tgagtggaga actgacttta gagggcggtg ccctggtgct ggctgaccag ggcgtgtgct
     3841 gcattgatga gtttgacaag atggccgagg ctgaccgcac agccatccat gaggttatgg
     3901 agcagcagac catctccatc gccaaggcgg ggattctcac cacgctcaat gcccgatgct
     3961 ccatcctggc tgcggccaat cctgcctacg gccgatataa tccccgccgc agtctggagc
     4021 agaacgtcca gcttccggct gctctgcttt ctcgattcga cctcctctgg ctgattcaag
     4081 accggcccga cagagacaat gacctccggt gaatgaagta gatgacaccc atagagacag
     4141 cccattttag tgactttggg gcagggaggt aaaatcagaa agccactcaa cttgtctgtg
     4201 tgcagacacc ttaaacacct ttccttgttt gcagttcata tggagatagg ttggagctaa
     4261 gatcttgagg gttttctggg aggcctgtct gccatgcttt ggagaagagg tggtgctaaa
     4321 aggggatact gagtcaagat tctcagggtg ttagagctct aaagactgac gcccgtcttc
     4381 tctccaggtt ggcccagcac atcacctatg tccaccagca cagccggcag ccccctgccc
     4441 aatttgaacc tttggacatg aaacttatga ggtaggaggg gtggagatgg gtatgtctgg
     4501 ggagagtact ggtctgtggc attgtcttat gccaagtgtg tctggaaagc attgagtctt
     4561 gaaaggactc atctgcttat ctatttcagc ctcaaaagta gctgagcctg ggtctctctg
     4621 tagccaaggg atcatcagaa actttttttc tgttttggga tgacagagca gcaggcctta
     4681 tataatccta gctactctga aacctgagtc attttgggga tgtctccaaa aactacttcc
     4741 ctcctgtata taaaggaacc tctctcactc tctaaatcct gtccgaggca ggtctagctt
     4801 tttggccata agtgcttcca tggcacagat gactcgggtt tctaaactgg ttttcacatg
     4861 gtctctgaag tctgtcaagg ggttaagtta aatcctccca ctacaggtgc tttggtaaat
     4921 gtcattctcc tgaaaagttg gaagttactt gaagtgtcct ggcagccctg ttttggagaa
     4981 atccctcact gtccacgtca gcatagaata ttaagtgggg aatctcataa agcctgcctg
     5041 ctttcccgct ttcccatgaa ccttgtttcc cacagacggt acatagccat gtgccacgag
     5101 agacagccca cagtgcctga atctctggct gactacatca ccgcagcata tgtggagatg
     5161 aggcgagagg cccgggccag taaggatgcc acctatactt ctgcccggac cctgctggcc
     5221 attctccgac tttccactgc tctggtgagt ggtgggtccc tgtcagctgg aaagctgacc
     5281 cctgcctgcc acctcaccta atgaccctca agccgcctcc ttccctccta ccaggtcgag
     5341 cttgatatcg aattccgaag ttcctattct ctagaaagta taggaacttc aggtctgaag
     5401 aggagtttac gtccagccaa gctagcttgg ctgcaggtcg tcgaaattct accgggtagg
     5461 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     5521 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     5581 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     5641 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     5701 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     5761 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     5821 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     5881 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     5941 tccgggcctt tcgacctgca gcctgttgac aattaatcat cggcatagta tatcggcata
     6001 gtataatacg acaaggtgag gaactaaacc atgggatcgg ccattgaaca agatggattg
     6061 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     6121 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     6181 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     6241 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6301 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6361 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6421 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     6481 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     6541 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc
     6601 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     6661 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     6721 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     6781 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat
     6841 caattctcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6901 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6961 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7021 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7081 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgac tagagcttgc
     7141 ggaacccttc gaagttccta ttctctagaa agtataggaa cttcatcagt ctcgacccaa
     7201 cactgcagat gtgggggggc agggaggagt ccccattttt gttcaatgag taggcaaatg
     7261 tggcttgccc aggcttgaag ctggagcaaa gaaagatcct tgacttcatt cccctcctcc
     7321 cctcctatcc caatcttttc tatgccttcc ccttaggctc gacttcggat ggtagacatt
     7381 gtggagaagg aagatgtaaa tgaagccata aggttgatgg agatgtcgaa ggactccctt
     7441 ctaggtgaaa agggacagac agctaggtga ggattttaat gaagagtgag actggaccac
     7501 tgggcttatt gacctggctt gccctcaggg ttttgatgct gattgctgct actgtttgtg
     7561 gccttgcacc ttcacctttt ccatccttgt cttttaggac ccagaggcca gcagatgtga
     7621 tatttgccac catccgtgaa ttggtgtcac gggggcggag tgtccacttt tctgaggctg
     7681 aacagcgctg catatctcgt ggcttcacac cagcccagtt ccaggcagct ctggatgaat
     7741 atgaggagct aaatgtctgg caggttaata cttctcggac acggatcacc tttgtctgat
     7801 agccagtttt tacaccctcc tgcctacacc agagggcatg ctgaatgccc cctcaagaaa
     7861 aaatctcatt ctcccatgct gcactttttt attctgccaa taaagtgttt tgattgcctc
     7921 ttgcttgtta ataactctgt agagttttta gtatgctctc tcctccccta gtctctagta
     7981 ctgaccctta ctatgtgcta gtcaatctgg gagatagaag gccttgggac cactgtttct
     8041 tgagatgctt ccaattatgg acatttcgtt tgttagaccc ccacatctag acgtgatgac
     8101 accaggatgt gaagggcaga aagttgcgcc tagcacaagg tatgacttaa gtggaaggcc
     8161 taaggatgag gtacatatca ccctcctagg taccttcagg atatagaaaa acaaggactt
     8221 gagataacca caattacaaa gatggaattc agcttccctc cccttcctat ttgagaaaca
     8281 tttgcagtga aattaaatta gcctgggaaa gcaatctttc tgtggagctg tgacatgaat
     8341 tgatcacaga gcacaaaaac tggaagcaag caggtcgtcc tatcacattt tgtctacttc
     8401 aacaaggggt cacctcgagt cattaaaagg tgggtaggtg gtcaaaaaat gtggattaat
     8461 gggtgaagct tccttaagag tccatgttga gaaaccagtg tggtgcctga gaggacaaag
     8521 ccactggaag ggctgctaag gggctgctct tttattaatg agtttctcta aagggaatgt
     8581 ggtactcagg tccatggaac agctgtctga gccccacaca ccatcccttc cagaaccatc
     8641 atcagaaaaa cagtccccgc attcgccggc caatgccttg tcggtcgtag aggacgttga
     8701 acttgttcac aaactggttc attgtgttgc acgttttggt gatggtgccg aggtaggcca
     8761 tgagccccac gtcattgcat tgctgggagg gaacacaaag aaccagttcc ccaggggtcc
     8821 ctcgtattag ccagtgacac ttgagtttca cctcctacct gtgaggtggt gtgagcccca
     8881 cctccccatc aaaggtgtca acactcacat cataaaagtc tgtcttgaac ttgtcagtgc
     8941 tgagaactgg aagacagtga catagggcat aggcctcccg caggatctca tggttgaagg
     9001 gaacctctcc taaggacaca aggaggctgc actgatgtgg atgctgtcac ttttccagga
     9061 gaggaaaaat gtttcaaagt gacaggcacc tctcctacct gcttcagagg ccttgacata
     9121 ttctaaaatg agcttcacac ggctgtgcag catcttgatg gcactatgct gagctatcag
     9181 gtgttcagcc actgcagggg aatggggcaa atgaagggcg gcctgtggcc acacccaacc
     9241 cctacctcca aagactgaat tcccctccct taccagtgga gttctcccca ctgcctgttg
     9301 ctgtcatccg ggccacgtgg tctacaccga tccgttcagc ttcctcagtg gccagagtgt
     9361 aagtgagctc agcaaacagc attgtggcct gcagagtaaa gagttctcag atagggagag
     9421 tcaggaaaca gacctaggcc taggcagatg gctcagcagg tagagtactt gctgcacaac
     9481 tgaggatctc aatttagagc ctcggaaccc acattaagtg agatgtgaca gcatgtgact
     9541 gtaatcctgg aacccctaca gcaagatggg aagtggagac acaattccca gaagctcaca
     9601 ggccaacctg cctggcaaac aagtgagtct gtcccaaggt agaaagctag gatggacacc
     9661 cagggttgtc ctatgcctgc acacggcatg caggaaacag gctagggccc ggtacccagc
     9721 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     9781 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9841 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9901 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9961 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    10021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    10081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    10141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    10621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10861 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10921 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10981 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    11041 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    11101 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    11161 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11221 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11281 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11341 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11401 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11461 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11521 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11581 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11641 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11701 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11761 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11821 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11881 ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
