LOCUS       sequence-89210-e        3307 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3307)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..3307
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1849..1858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1855..3285
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag accgccatgg aagggaagtg gttgctgtgt
       61 atgttactgg tgcttggaac tgctattgtt gaggctcatg atggacatga tgatgatgtg
      121 attgatattg aggatgacct tgacgatgtc attgaagagg tagaagactc aaaaccagat
      181 accactgctc ctccttcatc tcccaaggtt acttacaaag ctccagttcc aacaggggaa
      241 gtatattttg ctgattcttt tgacagagga actctgtcag ggtggatttt atccaaagcc
      301 aagaaagacg ataccgatga tgaaattgcc aaatatgatg gaaagtggga ggtagaggaa
      361 atgaaggagt caaagcttcc aggtgataaa ggacttgtgt tgatgtctcg ggccaagcat
      421 catgccatct ctgctaaact gaacaagccc ttcctgtttg acaccaagcc tctcattgtt
      481 cagtatgagg ttaatttcca aaatggaata gaatgtggtg gtgcctatgt gaaactgctt
      541 tctaaaacac cagaactcaa cctggatcag ttccatgaca agacccctta tacgattatg
      601 tttggtccag ataaatgtgg agaggactat aaactgcact tcatcttccg acacaaaaac
      661 cccaaaacgg gtatctatga agaaaaacat gctaagaggc cagatgcaga tctgaagacc
      721 tattttactg ataagaaaac acatctttac acactaatct tgaatccaga taatagtttt
      781 gaaatactgg ttgaccaatc tgtggtgaat agtggaaatc tgctcaatga catgactcct
      841 cctgtaaatc cttcacgtga aattgaggac ccagaagacc ggaagcccga ggattgggat
      901 gaaagaccaa aaatcccaga tccagaagct gtcaagccag atgactggga tgaagatgcc
      961 cctgctaaga ttccagatga agaggccaca aaacccgaag gctggttaga tgatgagcct
     1021 gagtacgtac ctgatccaga cgcagagaaa cctgaggatt gggatgaaga catggatgga
     1081 gaatgggagg ctcctcagat tgccaaccct agatgtgagt cagctcctgg atgtggtgtc
     1141 tggcagcgac ctgtgattga caaccccaat tataaaggca aatggaagcc tcctatgatt
     1201 gacaatccca gttaccaggg aatctggaaa cccaggaaaa taccaaatcc agatttcttt
     1261 gaagatctgg aacctttcag aatgactcct tttagtgcta ttggtttgga gctgtggtcc
     1321 atgacctctg acattttttt tgacaacttt atcatttgtg ctgatcgaag aatagttgat
     1381 gattgggcca atgatggatg gggcctgaag aaagctgctg atggggctgc tgagccaggc
     1441 gttgtggggc agatgaacga ggcagctgaa gagcgcccgt ggctgtgggt agtctatatt
     1501 ctaactgtag cccttcctgt gttcctggtt atcctcttct gctgttctgg aaagaaacag
     1561 accagtggta tggagtataa gaaaactgat gcacctcaac cggatgtgaa ggaagaggaa
     1621 gaagagaagg aagaggaaaa ggacaaggga gatgaggagg aggaaggaga agagaaactt
     1681 gaagagaaac agaaaagtga tgctgaagaa gatggtggca ctgtcagtca agaggaggaa
     1741 gacagaaaac ctaaagcaga ggaggatgaa attttgaaca gatcaccaag aaacagaaag
     1801 ccacgaagag aggggggtgc gtcaggcggc agcggatctg gaccggtcgc caccatggtg
     1861 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     1921 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1981 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     2041 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     2101 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2161 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     2221 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     2281 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     2341 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     2401 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     2461 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     2521 cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc
     2581 tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     2641 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2701 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     2761 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     2821 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2881 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     2941 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     3001 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     3061 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     3121 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     3181 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     3241 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaagcggc cgcgactcta
     3301 gatcata
//
