LOCUS       sequence-89211-e        2806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2806)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Calreticulin-N-16
FEATURES             Location/Qualifiers
     source          1..2806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1338..1347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1344..2774
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gttcagatcc gctagcgcta ccggactcag atctcgaggc caccatgctg ctatccgtgc
       61 cgctgctgct cggcctcctc ggcctggccg tcgccgagcc tgccgtctac ttcaaggagc
      121 agtttctgga cggagacggg tggacttccc gctggatcga atccaaacac aagtcagatt
      181 ttggcaaatt cgttctcagt tccggcaagt tctacggtga cgaggagaaa gataaaggtt
      241 tgcagacaag ccaggatgca cgcttttatg ctctgtcggc cagtttcgag cctttcagca
      301 acaaaggcca gacgctggtg gtgcagttca cggtgaaaca tgagcagaac atcgactgtg
      361 ggggcggcta tgtgaagctg tttcctaata gtttggacca gacagacatg cacggagact
      421 cagaatacaa catcatgttt ggtcccgaca tctgtggccc tggcaccaag aaggttcatg
      481 tcatcttcaa ctacaagggc aagaacgtgc tgatcaacaa ggacatccgt tgcaaggatg
      541 atgagtttac acacctgtac acactgattg tgcggccaga caacacctat gaggtgaaga
      601 ttgacaacag ccaggtggag tccggctcct tggaagacga ttgggacttc ctgccaccca
      661 agaagataaa ggatcctgat gcttcaaaac cggaagactg ggatgagcgg gccaagatcg
      721 atgatcccac agactccaag cctgaggact gggacaagcc cgagcatatc cctgaccctg
      781 atgctaagaa gcccgaggac tgggatgaag agatggacgg agagtgggaa cccccagtga
      841 ttcagaaccc tgagtacaag ggtgagtgga agccccggca gatcgacaac ccagattaca
      901 agggcacttg gatccaccca gaaattgaca accccgagta ttctcccgat cccagtatct
      961 atgcctatga taactttggc gtgctgggcc tggacctctg gcaggtcaag tctggcacca
     1021 tctttgacaa cttcctcatc accaacgatg aggcatacgc tgaggagttt ggcaacgaga
     1081 cgtggggcgt aacaaaggca gcagagaaac aaatgaagga caaacaggac gaggagcaga
     1141 ggcttaagga ggaggaagaa gacaagaaac gcaaagagga ggaggaggca gaggacaagg
     1201 aggatgatga ggacaaagat gaggatgagg aggatgagga ggacaaggag gaagatgagg
     1261 aggaagatgt ccccggccag gccaaggacg agctggcatc cgggggtgcg tcaggcggca
     1321 gcggatctgg accggtcgcc accatggtga gcaagggcga ggaggtcatc aaagagttca
     1381 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
     1441 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
     1501 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt
     1561 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca
     1621 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct
     1681 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg
     1741 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
     1801 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc
     1861 actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct
     1921 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     1981 aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc accggcagca
     2041 ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc gtcatcaaag
     2101 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
     2161 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     2221 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
     2281 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
     2341 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
     2401 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
     2461 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     2521 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
     2581 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
     2641 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
     2701 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg
     2761 agctgtacaa gtaagcggcc gcgactctag atcataatca gccata
//
