LOCUS       sequence-89212-e        2548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2548)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..2548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1073..1082
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1079..2509
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattccag
       61 gatgtggtgg tccttgatcc ctctctcttg cctgctggca ctgaccagtg cccatgacaa
      121 gccttccttt cacccactgt cggacgacat gattaactat atcaacaaac agaatacaac
      181 atggcaggct ggacgcaact tctacaatgt tgacataagc tatctgaaga agctgtgtgg
      241 aactgtcctg ggtggaccca agctgccgga aagggttggg ttcagcgagg acataaatct
      301 acctgaatcc tttgatgcac gggaacagtg gtccaattgc ccgaccatcg cacagatcag
      361 agaccagggg tcctgtggct cttgttgggc atttggggca gtggaagcca tgtctgaccg
      421 aatctgcatt cacaccaatg gccgagtcaa tgtggaggtg tctgctgagg acctgcttac
      481 ctgctgtggt atccagtgtg gggatggctg taatggtggc tatccctctg gagcatggaa
      541 cttctggact agaaaaggcc tggtttctgg tggagtatac aattctcata taggctgctt
      601 accctacacc atccctccct gtgaacacca tgtcaatggc tcccgtcccc catgcactgg
      661 agaaggagat actcccaagt gcaacaagat gtgtgaggct ggctactcca catcctacaa
      721 ggaagataag cactatgggt acacttccta cagtgtgtct gacagcgaga aggagatcat
      781 ggcggaaatc tacaaaaatg gcccagtgga gggtgctttt actgtgtttt ctgacttctt
      841 gacttacaaa tcaggcgtat acaagcatga agccggtgat gtgatgggag gccatgccat
      901 ccgcattctg ggctggggaa tagagaatgg agtaccctac tggctggtag caaactcctg
      961 gaacgttgac tggggtgata atggtttctt taaaatcctc agaggagaga accactgtgg
     1021 aattgaatca gaaatcgtgg ctggaatccc acgcactcag gatccaccgg tcgccaccat
     1081 ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     1141 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     1201 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     1261 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     1321 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     1381 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     1441 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     1501 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     1561 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     1621 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga
     1681 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     1741 ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc
     1801 ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat
     1861 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     1921 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     1981 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     2041 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2101 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     2161 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     2221 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     2281 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     2341 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     2401 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     2461 gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaag cggccgcgac
     2521 tctagatcat aatcagccat accacatt
//
