LOCUS       sequence-89213-e        2257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2257)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-CD9-10
FEATURES             Location/Qualifiers
     source          1..2257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..1453
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggtca tcaaagagtt
       61 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
      121 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      181 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
      241 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
      301 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
      361 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
      421 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
      481 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
      541 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
      601 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
      661 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
      721 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
      781 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
      841 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      901 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
      961 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     1021 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     1081 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     1141 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     1201 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     1261 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     1321 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     1381 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     1441 cgagctgtac aagtccggac tcagatctcg agctcaagct tccccggtca aaggaggcac
     1501 caagtgcatc aaatacctgc tgttcggatt taacttcatc ttctggcttg ccgggattgc
     1561 tgtccttgcc attggactat ggctccgatt cgactctcag accaagagca tcttcgagca
     1621 agaaactaat aataataatt ccagcttcta cacaggagtc tatattctga tcggagccgg
     1681 cgccctcatg atgctggtgg gcttcctggg ctgctgcggg gctgtgcagg agtcccagtg
     1741 catgctggga ctgttcttcg gcttcctctt ggtgatattc gccattgaaa tagctgcggc
     1801 catctgggga tattcccaca aggatgaggt gattaaggaa gtccaggagt tttacaagga
     1861 cacctacaac aagctgaaaa ccaaggatga gccccagcgg gaaacgctga aagccatcca
     1921 ctatgcgttg aactgctgtg gtttggctgg gggcgtggaa cagtttatct cagacatctg
     1981 ccccaagaag gacgtactcg aaaccttcac cgtgaagtcc tgtcctgatg ccatcaaaga
     2041 ggtcttcgac aataaattcc acatcatcgg cgcagtgggc atcggcattg ccgtggtcat
     2101 gatatttggc atgatcttca gtatgatctt gtgctgtgct atccgcagga accgcgagat
     2161 ggtctaggga tccaagggcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
     2221 gatctagata actgatcata atcagccata ccacatt
//
