LOCUS       sequence-89215-e        2279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2279)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-CD81-10
FEATURES             Location/Qualifiers
     source          1..2279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..1456
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga
       61 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
      121 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      181 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
      241 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
      301 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
      361 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
      421 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
      481 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
      541 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
      601 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
      661 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
      721 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
      781 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      841 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      901 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      961 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     1021 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     1081 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     1141 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     1201 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     1261 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     1321 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     1381 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     1441 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttccggag tggagggctg
     1501 caccaagtgc atcaagtacc tgctcttcgt cttcaatttc gtcttctggc tggctggagg
     1561 cgtgatcctg ggtgtggccc tgtggctccg ccatgacccg cagaccacca acctcctgta
     1621 tctggagctg ggagacaagc ccgcgcccaa caccttctat gtaggcatct acatcctcat
     1681 cgctgtgggc gctgtcatga tgttcgttgg cttcctgggc tgctacgggg ccatccagga
     1741 atcccagtgc ctgctgggga cgttcttcac ctgcctggtc atcctgtttg cctgtgaggt
     1801 ggccgccggc atctggggct ttgtcaacaa ggaccagatc gccaaggatg tgaagcagtt
     1861 ctatgaccag gccctacagc aggccgtggt ggatgatgac gccaacaacg ccaaggctgt
     1921 ggtgaagacc ttccacgaga cgcttgactg ctgtggctcc agcacactga ctgctttgac
     1981 cacctcagtg ctcaagaaca atttgtgtcc ctcgggcagc aacatcatca gcaacctctt
     2041 caaggaggac tgccaccaga agatcgatga cctcttctcc gggaagctgt acctcatcgg
     2101 cattgctgcc atcgtggtcg ctgtgatcat gatcttcgag atgatcctga gcatggtgct
     2161 gtgctgtggc atccggaaca gctccgtgta ctgaggcgga tccaagggcg aattctgcag
     2221 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccat
//
