LOCUS       sequence-89216-e        2322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2322)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-CD151-7
FEATURES             Location/Qualifiers
     source          1..2322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..1461
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggtcatc
       61 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
      121 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      181 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
      241 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
      301 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
      361 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
      421 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
      481 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
      541 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
      601 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
      661 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc
      721 accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc
      781 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag
      841 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      901 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg
      961 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc
     1021 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc
     1081 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc
     1141 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     1201 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag
     1261 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
     1321 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag
     1381 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac
     1441 ggcatggacg agctgtacaa gtccggactc agatctggcg gtatgggtga gttcaacgag
     1501 aagaagacaa catgtggcac cgtttgcctc aagtacctgc tgtttaccta caattgctgc
     1561 ttctggctgg ctggcctggc tgtcatggca gtgggcatct ggacgctggc cctcaagagt
     1621 gactacatca gcctgctggc ctcaggcacc tacctggcca cagcctacat cctggtggtg
     1681 gcgggcactg tcgtcatggt gactggggtc ttgggctgct gcgccacctt caaggagcgt
     1741 cggaacctgc tgcgcctgta cttcatcctg ctcctcatca tctttctgct ggagatcatc
     1801 gctggtatcc tcgcctacgc ctactaccag cagctgaaca cggagctcaa ggagaacctg
     1861 aaggacacca tgaccaagcg ctaccaccag ccgggccatg aggctgtgac cagcgctgtg
     1921 gaccagctgc agcaggagtt ccactgctgt ggcagcaaca actcacagga ctggcgagac
     1981 agtgagtgga tccgctcaca ggaggccggt ggccgtgtgg tcccagacag ctgctgcaag
     2041 acggtggtgg ctctttgtgg acagcgagac catgcctcca acatctacaa ggtggagggc
     2101 ggctgcatca ccaagttgga gaccttcatc caggagcacc tgagggtcat tggggctgtg
     2161 gggatcggca ttgcctgtgt gcaggtcttt ggcatgatct tcacgtgctg cctgtacagg
     2221 agtctcaagc tggagcacta ctgagaattc tgcagtcgac ggtaccgcgg gcccgggatc
     2281 caccggatct agataactga tcataatcag ccataccaca tt
//
