LOCUS       sequence-89217-e        1866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1866)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-CEBPA237-C-4
FEATURES             Location/Qualifiers
     source          1..1866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..1455
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggaggt catcaaagag
       61 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
      121 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      181 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
      241 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
      301 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
      361 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
      421 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
      481 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
      541 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
      601 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
      661 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc
      721 agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc
      781 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
      841 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      901 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
      961 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     1021 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     1081 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     1141 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     1201 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     1261 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     1321 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     1381 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg
     1441 gacgagctgt acaagtccgg acctgcaatg ggtgctgcgg gcctgccggg ccccgggggc
     1501 tcgctcaagg gcttggctgg tccgcacccc gacctccgca ccggcggcgg cggcggcggc
     1561 ggggccggcg cgggcaaggc caagaagtcg gtggataaga acagcaacga gtaccgggta
     1621 cggcgggaac gcaacaacat cgcggtgcgc aagagccgag ataaagccaa acagcgcaac
     1681 gtggagacgc agcagaaggt gttggagttg accagtgaca atgaccgcct gcgcaagcgg
     1741 gtggaacagc tgagccgtga actggacacg ctgcggggta tcttccgcca gctgcctgag
     1801 agctccttgg tcaaggccat gggcaactgc gcgtgaggcg cgcggatcca ccggatctag
     1861 ataact
//
