LOCUS       sequence-89218-e        2059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Gao L, Shao L, Higgins CD, Poulton JS, Peifer M, Davidson MW, Wu X, 
            Goldstein B, Betzig E
  TITLE     Noninvasive imaging beyond the diffraction limit of 3D dynamics in 
            thickly fluorescent specimens.
  JOURNAL   Cell. 2012 Dec 7;151(6):1370-85. doi: 10.1016/j.cell.2012.10.008.
  PUBMED    23217717
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.10"; journalName: "Cell"; date: "2012-12-7- 7";
            volume: "151"; issue: "6"; pages: "1370-85"
COMMENT     Sequence Label: tdTomato-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..2059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    527..579
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      587..596
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             593..2023
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gtcagatccg ctagccacca tgggccccaa gcggcggcag ctgacgttcc gggagaagtc
       61 gcggatcatc caggaggtgg aggagaaccc ggacctacgc aagggcgaga tcgcgcggcg
      121 cttcaacatc ccgccgtcca cgctgagcac gatcctgaag aacaagcgcg ccatcctggc
      181 gtcggagcgc aagtacgggg tggcctccac ctgccgcaag accaacaagc tgtctcccta
      241 cgacaagctc gagggcttgc tcatcgcctg gttccagcag atccgcgccg ccggcctgcc
      301 ggtcaagggc atcatcctca aggagaaggc gctgcgcata gccgaggagc tgggcatgga
      361 cgacttcacc gcctccaacg gctggctgga ccgcttccgc cggcgccacg gcgtggtgtc
      421 ctgcagcggc gtggcccgcg cccgcgcgcg aaacgctgcc ccccgcaccc cggcggcgcc
      481 tgccagtccg gccgcggtgc cctcggaggg cagtggcggg agcactctcg agctcaagct
      541 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
      601 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
      661 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      721 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
      781 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
      841 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      901 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
      961 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     1021 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     1081 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     1141 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     1201 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     1261 cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc
     1321 cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     1381 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     1441 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     1501 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     1561 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     1621 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     1681 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     1741 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     1801 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     1861 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga
     1921 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     1981 ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagcggccg cgactctaga
     2041 tcataatcag ccataccac
//
