LOCUS       sequence-89220-e        3144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3144)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-C-Src-7
FEATURES             Location/Qualifiers
     source          1..3144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..96
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      1669..1678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1675..3105
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagct caagcttcga attcatgggg
       61 agcagcaaga gcaagcccaa ggaccccagc cagcgccggc gcagcctgga gccacccgac
      121 agcacccacc acgggggatt cccagcctcg cagaccccca acaagacagc agcccccgac
      181 acgcaccgca cccccagccg ctcctttggg accgtggcca ccgagcccaa gctcttcggg
      241 ggcttcaaca cttctgacac cgtcacgtcg ccgcagcgtg ccggggcact ggctggcggc
      301 gtcaccactt tcgtggctct ctatgactac gagtcccgga ctgaaacgga cttgtccttc
      361 aagaaaggag aacgcctgca gattgtcaac aacacggaag gtgactggtg gctggctcat
      421 tccctcacta caggacagac gggctacatc cccagtaact atgtcgcgcc ctcagactcc
      481 atccaggctg aagagtggta ctttgggaag atcactcgtc gggagtccga gcggctgctg
      541 ctcaaccccg aaaacccccg gggaaccttc ttggtccggg agagcgagac gacaaaaggt
      601 gcctattgcc tctccgtttc tgactttgac aacgccaagg ggctcaatgt gaagcactac
      661 aagatccgca agctggacag cggcggcttc tacatcacct cacgcacaca gttcagcagc
      721 ctgcagcagc tggtggccta ctactccaaa catgctgatg gcttgtgcca ccgcctgacc
      781 aacgtctgcc ccacgtccaa gccccagacc cagggactcg ccaaggacgc gtgggaaatc
      841 ccccgggagt cgctgcggct ggaggtgaag ctggggcagg gctgctttgg agaggtctgg
      901 atggggacct ggaacggcac caccagagtg gccataaaga ctctgaagcc cggcaccatg
      961 tccccggagg ccttcctgca ggaagcccaa gtgatgaaga agctccggca tgagaagctg
     1021 gttcagctgt acgcagtggt gtcggaagag cccatctaca tcgtcactga gtacatgagc
     1081 aaggggagcc tcctggattt cctgaaggga gagatgggca agtacctgcg gctgccacag
     1141 ctcgtcgata tggctgctca gattgcatcc ggcatggcct atgtggagag gatgaactac
     1201 gtgcaccgag acctgcgggc ggccaacatc ctggtggggg agaacctggt gtgcaaggtg
     1261 gctgactttg ggctggcacg cctcatcgag gacaacgagt acacagcacg gcaaggtgcc
     1321 aagttcccca tcaagtggac agcccccgag gcagccctct atggccggtt caccatcaag
     1381 tcggatgtct ggtccttcgg catcctgctg actgagctga ccaccaaggg ccgggtgcca
     1441 tacccaggga tggtcaacag ggaggtgctg gaccaggtgg agaggggcta ccgcatgccc
     1501 tgcccgcccg agtgccccga gtcgctgcat gacctcatgt gccagtgctg gcggaaggac
     1561 cctgaggagc ggcccacttt tgagtacctg caggccttcc tggaggacta cttcacctcg
     1621 acagagcccc agtaccagcc tggagagaac ctacgggatc caccggtcgc caccatggtg
     1681 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     1741 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1801 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1861 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     1921 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1981 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     2041 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     2101 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     2161 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     2221 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     2281 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     2341 cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc
     2401 tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     2461 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2521 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     2581 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     2641 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2701 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     2761 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     2821 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     2881 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     2941 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     3001 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     3061 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaagcggc cgcgactcta
     3121 gatcataatc agccatacca catt
//
