LOCUS       sequence-89221-e        2272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2272)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Cx26-7
FEATURES             Location/Qualifiers
     source          1..2272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             804..2234
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcgcgg
       61 ccgcacgctc ctcgggacac agtgccaacc atccagagga caagatggat tggggcacac
      121 tacagagcat cctcgggggt gtcaacaagc actccaccag cattgggaaa atctggctca
      181 ctgtcctctt catcttccgc atcatgatcc tcgtggtggc cgcgaaggag gtgtggggag
      241 atgagcaagc cgattttgtt tgcaacactc tccagcctgg ctgtaagaat gtgtgctacg
      301 accactactt ccccatctct cacatccggc tctgggctct gcagctgatc atggtgtcca
      361 cgccggccct cctggtagct atgcacgtgg cctaccggag acacgaaaag aaacggaagt
      421 tcatgaaggg agagataaag aacgagttta aggacatcga agagatcaaa acccagaagg
      481 tccgtatcga agggtccctg tggtggacct acaccaccag catcttcttc cgggtcatct
      541 tcgaagctgt cttcatgtat gtcttttaca tcatgtacaa tggcttcttc atgcagcgtc
      601 tggtgaagtg taacgcctgg ccttgtccca atacagtgga ctgcttcatt tccaggccca
      661 cagaaaagac tgtcttcacg gtgttcatga tctctgtgtc tggaatttgc atcctgctaa
      721 acatcacaga gctgtgctat ctgttcatta ggtattgctc agggaagtcc aaaagaccag
      781 tcgcggatcc accggtcgcc accatggtga gcaagggcga ggaggtcatc aaagagttca
      841 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      901 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
      961 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt
     1021 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca
     1081 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct
     1141 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg
     1201 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
     1261 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc
     1321 actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct
     1381 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     1441 aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc accggcagca
     1501 ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc gtcatcaaag
     1561 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
     1621 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     1681 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
     1741 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
     1801 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
     1861 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
     1921 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     1981 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
     2041 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
     2101 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
     2161 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg
     2221 agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac at
//
