LOCUS       sequence-89222-e        2462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2462)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Cx32-7
FEATURES             Location/Qualifiers
     source          1..2462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             989..2419
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccctc
       61 tgggaaaggg cagcagcagc caggtgtggc agtgacaggg aggtgtgaat gaggcaggat
      121 gaactggaca ggtttgtaca ccttgctcag tggcgtgaac cggcattcta ctgccattgg
      181 ccgagtatgg ctctcggtca tcttcatctt cagaatcatg gtgctggtgg tggctgcaga
      241 gagtgtgtgg ggtgatgaga aatcttcctt catctgcaac acactccagc ctggctgcaa
      301 cagcgtttgc tatgaccaat tcttccccat ctcccatgtg cggctgtggt ccctgcagct
      361 catcctagtt tccaccccag ctctcctcgt ggccatgcac gtggctcacc agcaacacat
      421 agagaagaaa atgctacggc ttgagggcca tggggacccc ctacacctgg aggaggtgaa
      481 gaggcacaag gtccacatct cagggacact gtggtggacc tatgtcatca gcgtggtgtt
      541 ccggctgttg tttgaggccg tcttcatgta tgtcttttat ctgctctacc ctggctatgc
      601 catggtgcgg ctggtcaagt gcgacgtcta cccctgcccc aacacagtgg actgcttcgt
      661 gtcccgcccc accgagaaaa ccgtcttcac cgtcttcatg ctagctgcct ctggcatctg
      721 catcatcctc aatgtggccg aggtggtgta cctcatcatc cgggcctgtg cccgccgagc
      781 ccagcgccgc tccaatccac cttcccgcaa gggctcgggc ttcggccacc gcctctcacc
      841 tgaatacaag cagaatgaga tcaacaagct gctgagtgag caggatggct ccctgaaaga
      901 catactgcgc cgcagccctg gcaccggggc tgggctggct gaaaagagcg accgctgctc
      961 ggcctgcgcg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga
     1021 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     1081 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1141 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     1201 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     1261 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     1321 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     1381 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     1441 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     1501 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     1561 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     1621 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
     1681 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
     1741 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     1801 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     1861 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     1921 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     1981 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     2041 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     2101 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     2161 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     2221 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     2281 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     2341 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     2401 ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat accacattga
     2461 ga
//
