LOCUS       sequence-89225-e        2259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2259)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Dectin1A-N-10
FEATURES             Location/Qualifiers
     source          1..2259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      780..789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             786..2216
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 ccgctagcgc caccatggaa tatcatcctg atttagaaaa tttggatgaa gatggatata
       61 ctcaattaca cttcgactct caaagcaata ccaggatagc tgttgtttca gagaaaggat
      121 cgtgtgctgc atctcctcct tggcgcctca ttgctgtaat tttgggaatc ctatgcttgg
      181 taatactggt gatagctgtg gtcctgggta ccatggctat ttggagatcc aattcaggaa
      241 gcaacacatt ggagaatggc tactttctat caagaaataa agagaaccac agtcaaccca
      301 cacaatcatc tttagaagac agtgtgactc ctaccaaagc tgtcaaaacc acaggggttc
      361 tttccagccc ttgtcctcct aattggatta tatatgagaa gagctgttat ctattcagca
      421 tgtcactaaa ttcctgggat ggaagtaaaa gacaatgctg gcaactgggc tctaatctcc
      481 taaagataga cagctcaaat gaattgggat ttatagtaaa acaagtgtct tcccaacctg
      541 ataattcatt ttggataggc ctttctcggc cccagactga agtaccatgg ctctgggagg
      601 atggatcaac attctcttct aacttatttc agatcagaac cacagctacc caagaaaacc
      661 catctccaaa ttgtgtatgg attcacgtgt cagtcattta tgaccaactg tgtagtgtgc
      721 cctcatatag tatttgtgag aagaagtttt caatgagctc cggaggggat ccaccggtcg
      781 ccaccatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca
      841 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      901 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
      961 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     1021 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     1081 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     1141 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     1201 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     1261 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     1321 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     1381 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     1441 agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg
     1501 gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg
     1561 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1621 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     1681 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     1741 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     1801 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     1861 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     1921 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     1981 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     2041 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     2101 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     2161 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagtaagcgg
     2221 ccgcgactct agatcataat cagccatacc acattgaga
//
