LOCUS       sequence-89226-e        2398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 554, Emission: 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2398)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-EB3-7
FEATURES             Location/Qualifiers
     source          1..2398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             923..2353
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgagtcga ctgccaccat
       61 ggccgtcaat gtgtactcca catctgtgac cagtgaaaat ctgagtcgcc atgatatgct
      121 tgcatgggtc aacgactccc tgcacctcaa ctataccaag atagaacagc tttgttcagg
      181 ggcagcctac tgccagttca tggacatgct cttccccggc tgtgtgcact tgaggaaagt
      241 gaagttccag gccaaactag agcatgaata catccacaac ttcaaggtgc tgcaagcagc
      301 tttcaagaag atgggtgttg acaaaatcat tcctgtagag aaattagtga aaggaaaatt
      361 ccaagataat tttgagttta ttcagtggtt taagaaattc tttgacgcaa actatgatgg
      421 aaaggattac aaccctctgc tggcgcggca gggccaggac gtagcgccac ctcctaaccc
      481 aggtgatcag atcttcaaca aatccaagaa actcattggc acagcagttc cacagaggac
      541 gtcccccaca ggcccaaaaa acatgcagac ctctggccgg ctgagcaatg tggccccccc
      601 ctgcattctc cggaagaatc ctccatcagc ccgaaatggc ggccatgaga ctgatgccca
      661 aattcttgaa ctcaaccaac agctggtgga cttgaagctg acagtggatg ggctggagaa
      721 ggaacgtgac ttctacttca gcaaacttcg tgacatcgag ctcatctgcc aggagcatga
      781 aagtgaaaac agccctgtta tctcaggcat cattggcatc ctctatgcca cagaggaagg
      841 attcgcaccc cctgaggacg atgagattga agagcatcaa caagaagacc aggacgagta
      901 cctggatcca ccggtcgcca ccatggtgag caagggcgag gaggtcatca aagagttcat
      961 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     1021 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     1081 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     1141 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     1201 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     1261 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     1321 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     1381 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     1441 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     1501 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1561 acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac
     1621 cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga
     1681 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     1741 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1801 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     1861 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     1921 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     1981 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     2041 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     2101 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     2161 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     2221 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     2281 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga
     2341 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca ttgagagg
//
