LOCUS       sequence-89227-e        2210 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2210)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-ELP-1-25
FEATURES             Location/Qualifiers
     source          1..2210
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      735..744
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             741..2171
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 tcagatccgc tagcgccgcc accatggcca tgaacatttt ccggctgact ggggacctgt
       61 cccacctggc ggccatcgtc atcctgctgc tgaagatctg gaagacgcgc tcctgcgccg
      121 gtatttctgg gaaaagccag cttctgtttg cactggtctt cacaactcgt tacctggatc
      181 tttttacttc atttatttca ttgtataaca catctatgaa ggttatctac cttgcctgct
      241 cctatgccac agtgtacctg atctacctga aatttaaggc aacctacgat ggaaatcatg
      301 ataccttccg agtggagttt ctggtggtcc ctgtgggagg cctctcattt ttagttaatc
      361 acgatttctc tcctcttgag atcctctgga ccttctccat ctacctggag tccgtggcta
      421 tccttccgca gctgtttatg atcagcaaga ctggggaggc cgagaccatc accacccact
      481 acctgttctt cctgggcctc tatcgtgctt tgtatcttgt caactggatc tggcgcttct
      541 actttgaggg cttctttgac ctcattgctg tggtggccgg cgtagtccag accatcctat
      601 actgtgactt cttctacttg tacattacaa aagtactcaa gggaaagaag ctcagtttgc
      661 cagcaggcgg tgggggggag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc
      721 gggatccacc ggtcgccacc atggtgagca agggcgagga ggtcatcaaa gagttcatgc
      781 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
      841 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
      901 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
      961 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     1021 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     1081 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     1141 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     1201 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     1261 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     1321 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     1381 agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc ggcagcaccg
     1441 gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc atcaaagagt
     1501 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
     1561 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     1621 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
     1681 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
     1741 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
     1801 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
     1861 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     1921 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
     1981 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     2041 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
     2101 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc atggacgagc
     2161 tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
//
