LOCUS       sequence-89228-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..100
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     51..71
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             146..1573
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
       61 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga aaccgtcaga
      121 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggtca tcaaagagtt
      181 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
      241 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      301 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
      361 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
      421 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
      481 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
      541 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
      601 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
      661 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
      721 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
      781 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag
      841 caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa
      901 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
      961 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1021 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     1081 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     1141 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     1201 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     1261 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     1321 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     1381 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     1441 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     1501 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga
     1561 cgagctgtac aagtccggac tcagatctaa gctgaaccct cctgatgaga gtggccccgg
     1621 ctgcatgagc tgcaagtgtg tgctctcctg agaattctgc agtcgacggt accgcgggcc
     1681 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     1741 tacttgcttt aaaaaacctc ccacacctcc cccct
//
