LOCUS       sequence-89231-e        1675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear - Nucleolus, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1675)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-FTR-952
FEATURES             Location/Qualifiers
     source          1..1675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      197..206
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             203..1633
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttatgg gcatggcgcg
       61 accacggggc gcggggccct gcagccccgg gttggagcgg gctccgcgcc ggagcgtcgg
      121 ggagctgcgc ctgctcttcg aagcgcgctg cgccgcagtc gccgccgcag cggccgcagg
      181 gtcggatcca ccggtcgcca ccatggtgag caagggcgag gaggtcatca aagagttcat
      241 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
      301 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
      361 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
      421 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
      481 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
      541 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
      601 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
      661 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
      721 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
      781 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
      841 acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac
      901 cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga
      961 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     1021 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1081 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     1141 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     1201 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     1261 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     1321 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     1381 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     1441 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     1501 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     1561 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga
     1621 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca tgtag
//
