LOCUS       sequence-89232-e        2972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Associated Kinase, Excitation: 554, Emission:
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2972)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-FYN-N-10
FEATURES             Location/Qualifiers
     source          1..2972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1497..1506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1503..2933
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 ggacggtcag atccgctagc gccaccatgg gctgtgtgca atgtaaggat aaagaagcaa
       61 caaaactgac ggaggagagg gacggcagcc tgaaccagag ctctgggtac cgctatggca
      121 cagaccccac ccctcagcac taccccagct tcggtgtgac ctccatcccc aactacaaca
      181 acttccacgc agccgggggc caaggactca ccgtctttgg aggtgtgaac tcttcgtctc
      241 atacggggac cttgcgtacg agaggaggaa caggagtgac actctttgtg gccctttatg
      301 actatgaagc acggacagaa gatgacctga gttttcacaa aggagaaaaa tttcaaatat
      361 tgaacagctc ggaaggagat tggtgggaag cccgctcctt gacaactgga gagacaggtt
      421 acattcccag caattatgtg gctccagttg actctatcca ggcagaagag tggtactttg
      481 gaaaacttgg ccgaaaagat gctgagcgac agctattgtc ctttggaaac ccaagaggta
      541 cctttcttat ccgcgagagt gaaaccacca aaggtgccta ttcactttct atccgtgatt
      601 gggatgatat gaaaggagac catgtcaaac attataaaat tcgcaaactt gacaatggtg
      661 gatactacat taccacccgg gcccagtttg aaacacttca gcagcttgta caacattact
      721 caggtacctg gaatggaaac acaaaagtag ccataaagac tcttaaacca ggcacaatgt
      781 cccccgaatc attccttgag gaagcgcaga tcatgaagaa gctgaagcac gacaagctgg
      841 tccagctcta tgcagtggtg tctgaggagc ccatctacat cgtcaccgag tatatgaaca
      901 aaggaagttt actggatttc ttaaaagatg gagaaggaag agctctgaaa ttaccaaatc
      961 ttgtggacat ggcagcacag gtggctgcag gaatggctta catcgagcgc atgaattata
     1021 tccatagaga tctgcgatca gcaaacattc tagtggggaa tggactcata tgcaagattg
     1081 ctgacttcgg attggcccga ttgatagaag acaatgagta cacagcaaga caaggtgcaa
     1141 agttccccat caagtggacg gcccccgagg cagccctgta cgggaggttc acaatcaagt
     1201 ctgacgtgtg gtcttttgga atcttactca cagagctggt caccaaagga agagtgccat
     1261 acccaggcat gaacaaccgg gaggtgctgg agcaggtgga gcgaggctac aggatgccct
     1321 gcccgcagga ctgccccatc tctctgcatg agctcatgat ccactgctgg aaaaaggacc
     1381 ctgaagaacg ccccactttt gagtacttgc agagcttcct ggaagactac tttaccgcga
     1441 cagagcccca gtaccaacct ggtgaaaacc tggcatccag cggggatcca ccggtcgcca
     1501 ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     1561 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1621 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     1681 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     1741 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1801 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     1861 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     1921 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     1981 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     2041 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     2101 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     2161 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     2221 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     2281 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     2341 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     2401 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     2461 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2521 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     2581 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     2641 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     2701 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     2761 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     2821 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     2881 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagcggccg
     2941 cgactctaga tcataatcag ccataccaca tt
//
