LOCUS       sequence-89234-e        1616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1616)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-ER-5
FEATURES             Location/Qualifiers
     source          1..1616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..1517
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgctgc tatccgtgcc gttgctgctc ggcctcctcg
       61 gcctggccgt cgccgcaccg gtcgccacca tggtgagcaa gggcgaggag gtcatcaaag
      121 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
      181 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      241 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
      301 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
      361 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
      421 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
      481 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
      541 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
      601 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
      661 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
      721 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg
      781 gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca
      841 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
      901 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      961 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
     1021 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
     1081 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
     1141 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
     1201 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
     1261 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
     1321 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
     1381 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
     1441 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca
     1501 tggacgagct gtacaagtcc ggaaaggacg agctgtaaga attctgcagt cgacggtacc
     1561 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatt
//
