LOCUS       sequence-89235-e        2898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: tdTomato-Gamma-Tubulin-17
FEATURES             Location/Qualifiers
     source          1..2898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1423..1432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1429..2859
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgccg agggaaatca tcaccctaca gttgggccag
       61 tgcggcaatc agattgggtt cgagttctgg aaacagctgt gcgccgagca tggtatcagc
      121 cccgagggca tcgtggagga gttcgccacc gagggcactg accacaagga cgtctttttc
      181 taccaggcag acgatgagca ctacatcccc cgggccgtgc tgctggactt ggaaccccgg
      241 gtgatccact ccatcctcaa ctccccctat gccaagctct acaacccaga gaacatctac
      301 ctgtcggaac atggaggagg agctggcaac aactgggcca gcggattctc ccagggagaa
      361 aagatccatg aggacatttt tgacatcata gaccgggagg cagatggtag tgacagtcta
      421 gagggctttg tgctgtgtca ctccattgct ggggggacag gctctggact gggttcctac
      481 ctcttagaac ggctgaatga caggtatcct aagaagctgg tgcagacata ctcagtgttt
      541 cccaaccagg acgagatgag cgatgtggtg gtccagcctt acaattcact cctcacactc
      601 aagaggctga cgcagaatgc agactgtgtg gtggtgctgg acaacacagc cctgaaccgg
      661 attgccacag accgcctgca catccagaac ccatccttct cccagatcaa ccagctggtg
      721 tctaccatca tgtcagccag caccaccacc ctgcgctacc ctggctacat gaacaatgac
      781 ctcatcggcc tcatcgcctc gctcattccc accccacggc tccacttcct catgaccggc
      841 tacacccctc tcactacgga ccagtcagtg gccagcgtga ggaagaccac ggtcctggat
      901 gtcatgaggc ggctgctgca gcccaagaac gtgatggtgt ccacaggccg agaccgccag
      961 accaaccact gctacatcgc catcctcaac atcatccagg gagaggtgga ccccacccag
     1021 gtccacaaga gcttgcagag gatccgggaa cgcaagttgg ccaacttcat cccgtggggc
     1081 cccgccagca tccaggtggc cctgtcgagg aagtctccct acctgccctc ggcccaccgg
     1141 gtcagcgggc tcatgatggc caaccacacc agcatctcct cgctcttcga gagaacctgt
     1201 cgccagtatg acaagctgcg taagcgggag gccttcctgg agcagttccg caaggaggac
     1261 atgttcaagg acaactttga tgagatggac acatccaggg agattgtgca gcagctcatc
     1321 gatgagtacc atgcggccac acggccagac tacatctcct ggggcactca ggagcagcga
     1381 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
     1441 ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     1501 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1561 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     1621 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     1681 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     1741 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     1801 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     1861 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     1921 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     1981 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     2041 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     2101 ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag
     2161 gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     2221 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2281 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     2341 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     2401 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2461 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     2521 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     2581 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     2641 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     2701 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     2761 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     2821 cacctgttcc tgtacggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     2881 aatcagccat accacatt
//
