LOCUS       sequence-89236-e        1752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: tdTomato-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      277..286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             283..1713
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gtcagatccg ctagcatgag gcttcgggag ccgctcctga gcggcagcgc cgcgatgcca
       61 ggcgcgtccc tacagcgggc ctgccgcctg ctcgtggccg tctgcgctct gcaccttggc
      121 gtcaccctcg tttactacct ggctggccgc gacctgagcc gcctgcccca actggtcgga
      181 gtctccacac cgctgcaggg cggctcgaac agtgccgccg ccatcgggca gtcctccggg
      241 gagctccgga ccggaggggc caaggatcca ccggtcgcca ccatggtgag caagggcgag
      301 gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
      361 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      421 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
      481 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
      541 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
      601 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
      661 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      721 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
      781 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
      841 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
      901 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
      961 gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac
     1021 aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     1081 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1141 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     1201 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     1261 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     1321 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     1381 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     1441 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     1501 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     1561 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     1621 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     1681 ttcctgtacg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     1741 ccataccaca tt
//
