LOCUS       sequence-89239-e        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-H3.3-N-14
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      501..510
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             507..1937
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 aacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gccaccatgg
       61 cccgaaccaa gcagaccgct aggaagtcca ccggtgggaa agccccccgc aaacagctgg
      121 ccaccaaggc ggctcggaaa agcgcgccct ctaccggcgg ggtgaagaag cctcaccgct
      181 acaggccagg gaccgtggct ctgagagaga tccgtcgtta ccagaaatcg actgagctgc
      241 tcatccggaa gctgccattc cagagattgg tgagggagat cgcccaggat ttcaaaaccg
      301 acttgaggtt tcaaagtgca gccatcggtg cccttcagga ggctagcgaa gcatacctgg
      361 tggggttgtt tgaagatacc aatctgtgtg ccatccacgc caagagagtc accatcatgc
      421 ccaaagacat ccagttggct cgccggatac ggggggagag agctgggtcc ggaagcgcag
      481 gcagtgggga tccaccggtc gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt
      541 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
      601 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      661 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
      721 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
      781 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
      841 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
      901 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
      961 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
     1021 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     1081 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
     1141 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca
     1201 gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca
     1261 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     1321 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     1381 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     1441 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     1501 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     1561 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     1621 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     1681 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     1741 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     1801 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     1861 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
     1921 acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac cacatt
//
