LOCUS       sequence-89240-e        2925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2925)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-ILK-N-17
FEATURES             Location/Qualifiers
     source          1..2925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1449..1458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1455..2885
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gaccgccatg gacgacattt
       61 tcactcagtg ccgggagggc aacgcagtcg ccgttcgcct gtggctggac aacacggaga
      121 acgacctcaa ccagggggac gatcatggct tctccccctt gcactgggcc tgccgagagg
      181 gccgctctgc tgtggttgag atgttgatca tgcggggggc acggatcaat gtaatgaacc
      241 gtggggatga cacccccctg catctggcag ccagtcatgg acaccgtgat attgtacaga
      301 agctattgca gtacaaggca gacatcaatg cagtgaatga acatgggaat gtgcccctgc
      361 actatgcctg tttttggggc caagatcaag tggcagagga cctggtggca aatggggccc
      421 ttgtcagcat ctgtaacaag tatggagaga tgcctgtgga caaagccaag gcacccctga
      481 gagagcttct ccgagagcgg gcagagaaga tgggccagaa tctcaaccgt attccataca
      541 aggacacatt ctggaagggg accacccgca ctcggccccg aaatggaacc ctgaacaaac
      601 actctggcat tgacttcaaa cagcttaact tcctgacgaa gctcaacgag aatcactctg
      661 gagagctatg gaagggccgc tggcagggca atgacattgt cgtgaaggtg ctgaaggttc
      721 gagactggag tacaaggaag agcagggact tcaatgaaga gtgtccccgg ctcaggattt
      781 tctcgcatcc aaatgtgctc ccagtgctag gtgcctgcca gtctccacct gctcctcatc
      841 ctactctcat cacacactgg atgccatatg gatccctcta caatgtacta catgaaggca
      901 ccaatttcgt cgtggaccag agccaggctg tgaagtttgc tttggacatg gcaaggggca
      961 tggccttcct acacacacta gagcccctca tcccacgaca tgcactcaat agccgtagtg
     1021 taatgattga tgaggacatg actgcccgaa ttagcatggc tgatgtcaag ttctctttcc
     1081 aatgtcctgg tcgcatgtat gcacctgcct gggtagcccc cgaagctctg cagaagaagc
     1141 ctgaagacac aaacagacgc tcagcagaca tgtggagttt tgcagtgctt ctgtgggaac
     1201 tggtgacacg ggaggtaccc tttgctgacc tctccaatat ggagattgga atgaaggtgg
     1261 cattggaagg ccttcggcct accatcccac caggtatttc ccctcatgtg tgtaagctca
     1321 tgaagatctg catgaatgaa gaccctgcaa agcgacccaa atttgacatg attgtgccta
     1381 tccttgagaa gatgcaggac aagggaattc tgcagtcgac ggtaccgcgg gcccgggatc
     1441 caccggtcgc caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca
     1501 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     1561 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     1621 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     1681 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     1741 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     1801 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     1861 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     1921 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     1981 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     2041 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     2101 agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg
     2161 gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc
     2221 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     2281 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     2341 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     2401 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     2461 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     2521 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     2581 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     2641 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     2701 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     2761 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     2821 agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca
     2881 agtaagcggc cgcgactcta gatcataatc agccatacca cattt
//
