LOCUS       sequence-89244-e        2056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2056)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..2056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             586..2016
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttat gtcgagcaag
       61 agagccaagg ccaagaccac caagaagagg ccccagaggg ctacgtccaa tgtcttcgcc
      121 atgtttgacc agtcccagat ccaggagttt aaggaggcct tcaacatgat tgatcagaac
      181 cgcgatggct tcattgataa ggaggacctg cacgacatgt tggcctctct ggggaagaac
      241 cccacagacg agtacctgga gggcatgatg aacgaggcgc cagggcccat caacttcacc
      301 atgttcctca caatgtttgg ggagaagctg aacggcacag accccgagga tgtgatccgc
      361 aatgcctttg cctgctttga tgaggaggcc tcaggcttca tccacgagga ccacctgagg
      421 gagctgctca ccaccatggg cgaccgattc acggatgagg aggtggacga gatgtaccgc
      481 gaggcaccca ttgataagaa gggcaacttc aactacgtgg agttcactcg catcctcaaa
      541 cacggcgcca aggacaagga cgacctggat ccaccggtcg ccaccatggt gagcaagggc
      601 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
      661 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      721 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      781 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
      841 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
      901 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
      961 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     1021 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     1081 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     1141 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
     1201 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     1261 ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac
     1321 aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     1381 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1441 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     1501 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     1561 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1621 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     1681 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     1741 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     1801 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     1861 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     1921 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     1981 ctgttcctgt acggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat
     2041 cagccatacc acattt
//
