LOCUS       sequence-89245-e        2791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2791)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(41..103,104..1261)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1316..1325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1322..2752
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagcgccacc atggcggccc cgggcgcccg
       61 gcgtccgctg ctcctgttgc tgctggcagg ccttgcacac agcgccccag cactgttcga
      121 ggtgaaagac aacaacggca cagcgtgtat aatggccagc ttctctgcct cctttctgac
      181 cacctatgag gctggacatg tttctaaggt ctcgaatatg accctgccag cctctgcaga
      241 agtcctgaag aatagcagct cttgtggtga aaagaatgct tctgagccca ccctcgcaat
      301 cacctttgga gaaggatatt tactgaaact caccttcaca aaaaacacaa cacgttacag
      361 tgtccagcac atgtatttca catataacct gtcagacaca caattctttc ccaatgccag
      421 ctccaaaggg cccgacactg tggattccac aactgacatc aaggcagaca tcaacaaaac
      481 ataccgatgt gtcagcgaca tcagggtcta catgaagaat gtgaccattg tgctctggga
      541 cgctactatc caggcctacc tgccgagtag caacttcagc aaggaagaga cacgctgccc
      601 acaggatcaa ccttccccaa ctactgggcc acccagcccc tcaccaccac ttgtgcccac
      661 aaaccccagt gtgtccaagt acaatgtgac tggtgacaat ggaacctgcc tgctggcctc
      721 tatggcactg caactcaaca tcacctacat gaagaaggac aacacgactg tgaccagagc
      781 attcaacatc aacccaagtg acaaatatag tgggacttgc ggtgcccagt tggtgaccct
      841 gaaggtgggg aacaagagca gagtcctgga gctgcagttt gggatgaatg ccacttctag
      901 cctgtttttc ctgcaaggag ttcagttgaa catgactctt cctgatgcca tagagcccac
      961 gttcagcacc tccaactatt ccctgaaagc tcttcaggcc agtgtcggca actcatacaa
     1021 gtgcaacagt gaggagcaca tctttgtcag caaggcgctc gccctcaatg tcttcagcgt
     1081 gcaagtccag gctttcaggg tagaaagtga caggtttggg tctgtggaag agtgtgtaca
     1141 ggacggtaac aacatgctga tccccattgc tgtgggcggg gccctggcag ggctggtcct
     1201 catcgtcctc atcgcctacc tcatcggcag gaagaggagt cacgcgggct atcagaccat
     1261 ctcggaattc ggctccaccg gctccaccgg ctccaccggc gcggatccac cggtcgccac
     1321 catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga
     1381 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     1441 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     1501 catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat
     1561 ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     1621 cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     1681 ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     1741 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     1801 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     1861 catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct
     1921 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     1981 ccgccaccac ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac
     2041 cgcctcctcc gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg
     2101 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     2161 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     2221 ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc
     2281 cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     2341 gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac
     2401 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     2461 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     2521 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     2581 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac
     2641 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     2701 cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaagt aagcggccgc
     2761 gactctagat cataatcagc cataccacat t
//
